Name: Anti-NFIA Antibody (CAB3258)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB3258
Availability: OutOfStock

Antibody Name:	Anti-NFIA Antibody
Antibody SKU:	CAB3258
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 180-330 of human NFIA (NP_005586.1).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 180-330 of human NFIA (NP_005586.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AYFV HAAD SSQS ESPS QPSD ADIK DQPE NGHL GFQD SFVT SGVF SVTE LVRV SQTP IAAG TGPN FSLS DLES SSYY SMSP GAMR RSLP STSS TSST KRLK SVED EMDS PGEE PFYT GQGR SPGS GSQS SGWH EVEP GMPS PTTL KKSE KSG
Gene ID:	4774
Uniprot:	Q12857
Cellular Location:	Nucleus
Calculated MW:	54kDa/55kDa/60kDa
Observed MW:	60kDa

Synonyms:	NFIA, CTF, NF-I/A, NF1-A, NFI-A, NFI-L, BRMUTD
Background:	This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	NFI-A: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 1p31.3-p31.2 Cellular Component: nucleoplasm; nucleus; cell junction Molecular Function:transcription factor binding; transcription factor activity Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; viral genome replication; DNA replication; response to organic cyclic substance
NCBI Summary:	This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:	Q12857
NCBI GenInfo Identifier:	14194959
NCBI Gene ID:	4774
NCBI Accession:	Q12857.2
UniProt Secondary Accession:	Q12857,O00712, P08651, Q14938,
UniProt Related Accession:	Q12857
Molecular Weight:
NCBI Full Name:	Nuclear factor 1 A-type
NCBI Synonym Full Names:	nuclear factor I A
NCBI Official Symbol:	NFIA
NCBI Official Synonym Symbols:	CTF; NF1-A; NFI-A; NFI-L; BRMUTD; NF-I/A
NCBI Protein Information:	nuclear factor 1 A-type
UniProt Protein Name:	Nuclear factor 1 A-type
UniProt Synonym Protein Names:	CCAAT-box-binding transcription factor; CTF; Nuclear factor I/A; NF-I/A; NFI-A; TGGCA-binding protein
UniProt Gene Name:	NFIA
UniProt Entry Name:	NFIA_HUMAN

Western blot analysis of extracts of mouse brain, using NFIA antibody (CAB3258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

NFIA Antibody (PACO10831)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)