KO Validated Antibodies 1

Anti-NeuN Antibody (CAB0951)[KO Validated]

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SKU:
CAB0951
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-NeuN Antibody [KO Validated]
Antibody SKU:CAB0951
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NeuN (NP_001076044.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NeuN (NP_001076044.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAQP YPPA QYPP PPQN GIPA EYAP PPPH PTQD YSGQ TPVP TEHG MTLY TPAQ THPE QPGS EAST QPIA GTQT VPQT DEAA QTDS QPLH PSDP TEKQ QPKR LHVS NIPF RFRD PDLR QMFG QFGK ILDV EIIF NERG SKGF GFVT FETS SDAD RARE KLNG TIVE GRKI EVNN ATAR VMTN KKTG NPYT NGWK LNPV VGAV YGPE FYAV TGFP YPTT GTAV
Gene ID:146713
Uniprot:A6NFN3
Cellular Location:Cytoplasm, Nucleus
Calculated MW:33kDa/35kDa
Observed MW:50kDa
Synonyms:FOX-3, FOX3, HRNBP3, NEUN, NeuN, RBFOX3
Background:This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
UniProt Protein Function:NeuN: RNA-binding protein that regulates alternative splicing events.
UniProt Protein Details:

Protein type:RNA splicing; RNA processing; DNA-binding

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: cytoplasm; perikaryon; nucleus

Molecular Function:DNA binding; RNA binding; nucleotide binding

Biological Process: RNA splicing; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome

UniProt Code:A6NFN3
NCBI GenInfo Identifier:322510139
NCBI Gene ID:
NCBI Accession:A6NFN3.4
Molecular Weight:46-55kDa
NCBI Full Name:RNA binding protein fox-1 homolog 3
UniProt Protein Name:RNA binding protein fox-1 homolog 3
UniProt Synonym Protein Names:Fox-1 homolog C
UniProt Gene Name:RBFOX3  
UniProt Entry Name:RFOX3_HUMAN
Anti-NeuN Antibody [KO Validated] (CAB0951)Western blot analysis of extracts of various cell lines, using NeuN antibody (CAB0951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-NeuN Antibody [KO Validated] (CAB0951)Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] NeuN Rabbit pAb (CAB0951) at dilution of 1:100 (40x lens).
Anti-NeuN Antibody [KO Validated] (CAB0951)Immunofluorescence analysis of rat brain using [KO Validated] NeuN Rabbit pAb (CAB0951) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-NeuN Antibody [KO Validated] (CAB0951)Immunofluorescence analysis of mouse brain using [KO Validated] NeuN Rabbit pAb (CAB0951) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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