Neuroscience
Anti-Nestin Antibody (CAB11861)
- SKU:
- CAB11861
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Neuroscience
Description
| Antibody Name: | Anti-Nestin Antibody |
| Antibody SKU: | CAB11861 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1392-1621 of human Nestin (NP_006608.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, 293T, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1392-1621 of human Nestin (NP_006608.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QVPQ LLLD PAAW DRDG ESDG FADE EESG EEGE EDQE EGRE PGAG RWGP GSSV GSLQ ALSS SQRG EFLE SDSV SVSV PWDD SLRG AVAG APKT ALET ESQD SAEP SGSE EESD PVSL ERED KVPG PLEI PSGM EDAG PGAD IIGV NGQG PNLE GKSQ HVNG GVMN GLEQ SEEV GQGM PLVS EGDR GSPF QEEE GSAL KTSW AGAP VHLG QGQF LKFT QREG DRES WSSG ED |
| Gene ID: | 10763 |
| Uniprot: | P48681 |
| Cellular Location: | |
| Calculated MW: | 177kDa |
| Observed MW: | 180kDa |
| Synonyms: | NES, Nbla00170, nestin, Nestin |
| Background: | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. |
| UniProt Protein Function: | nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 1q23.1 Cellular Component: intermediate filament cytoskeleton; cytoplasm; intermediate filament Molecular Function:intermediate filament binding; structural molecule activity Biological Process: positive regulation of intermediate filament depolymerization; central nervous system development; negative regulation of catalytic activity; cell projection morphogenesis; brain development; negative regulation of neuron apoptosis; negative regulation of protein binding; G2/M transition of mitotic cell cycle; embryonic camera-type eye development |
| NCBI Summary: | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P48681 |
| NCBI GenInfo Identifier: | 146345464 |
| NCBI Gene ID: | 10763 |
| NCBI Accession: | P48681.2 |
| UniProt Secondary Accession: | P48681,O00552, Q3LIF5, Q5SYZ6, |
| UniProt Related Accession: | P48681 |
| Molecular Weight: | 1621 |
| NCBI Full Name: | Nestin |
| NCBI Synonym Full Names: | nestin |
| NCBI Official Symbol: | NES |
| NCBI Official Synonym Symbols: | Nbla00170 |
| NCBI Protein Information: | nestin |
| UniProt Protein Name: | Nestin |
| Protein Family: | Nestin |
| UniProt Gene Name: | NES |
| UniProt Entry Name: | NEST_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Nestin antibody (CAB11861) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using Nestin antibody (CAB11861) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-251MG cells using Nestin antibody (CAB11861) at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining. |
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