Epigenetics & Nuclear Signaling Antibodies 3
Anti-NELFE Antibody (CAB3249)
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- SKU:
- CAB3249
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-NELFE Antibody |
| Antibody SKU: | CAB3249 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, 293T, HepG2, SW480, Mouse liver, Mouse testis, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLVI PPGL SEEE EALQ KKFN KLKK KKKA LLAL KKQS SSST TSQG GVKR SLSE QPVM DTAT ATEQ AKQL VKSG AISA IKAE TKNS GFKR SRTL EGKL KDPE KGPV PTFQ PFQR SISA DDDL QESS RRPQ RKSL YESF VSSS DRLR ELGP DGEE AEGP GAGD GPPR SFDW GYEE RSGA HSSA SPPR SRSR DRSH ERNR DRDR DRER DRDR DRDR DRER DRDR DRDR DRDR ERDR DRER DRDR DREG PFRR SDSF PERR APRK GNTL YVYG EDMT PTLL RGAF |
| Gene ID: | 7936 |
| Uniprot: | P18615 |
| Cellular Location: | Nucleus |
| Calculated MW: | 40kDa/43kDa |
| Observed MW: | 43kDa |
| Synonyms: | NELFE, D6S45, NELF-E, RD, RDBP, RDP |
| Background: | The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. |
| UniProt Protein Function: | RDBP: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Belongs to the RRM NELF-E family. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Mitochondrial Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: nucleoplasm; mitochondrion; nucleus Molecular Function:protein binding; RNA binding; nucleotide binding Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression |
| NCBI Summary: | The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P18615 |
| NCBI GenInfo Identifier: | 1350554 |
| NCBI Gene ID: | 7936 |
| NCBI Accession: | P18615.3 |
| UniProt Related Accession: | P18615 |
| Molecular Weight: | 43kDa |
| NCBI Full Name: | Negative elongation factor E |
| NCBI Synonym Full Names: | negative elongation factor complex member E |
| NCBI Official Symbol: | NELFE |
| NCBI Official Synonym Symbols: | RD; RDP; RDBP; D6S45; NELF-E |
| NCBI Protein Information: | negative elongation factor E |
| UniProt Protein Name: | Negative elongation factor E |
| UniProt Synonym Protein Names: | RNA-binding protein RD |
| UniProt Gene Name: | NELFE |
| UniProt Entry Name: | NELFE_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NELFE antibody (CAB3249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat pancreas using NELFE Rabbit pAb (CAB3249) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophageal using NELFE Rabbit pAb (CAB3249) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using NELFE Rabbit pAb (CAB3249) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A-549 cells using NELFE antibody (CAB3249). |
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