Epigenetics & Nuclear Signaling Antibodies 1

Anti-NELFB Antibody (CAB12023)

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SKU:
CAB12023
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-NELFB Antibody
Antibody SKU:CAB12023
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 349-628 of human NELFB (NP_056271.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, LO2, 293T, SKOV3, HeLa, Mouse kidney, Mouse brain, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 349-628 of human NELFB (NP_056271.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LSMI LCDP FAIN TLAL STVR HLQE LVGQ ETLP RDSP DLLL LLRL LALG QGAW DMID SQVF KEPK MEVE LITR FLPM LMSF LVDD YTFN VDQK LPAE EKAP VSYP NTLP ESFT KFLQ EQRM ACEV GLYY VLHI TKQR NKNA LLRL LPGL VETF GDLA FGDI FLHL LTGN LALL ADEF ALED FCSS LFDG FFLT ASPR KENV HRHA LRLL IHLH PRVA PSKL EALQ KALE PTGQ SGEA VKEL YSQL GEKL EQLD HRKP SPAQ AAET PALE LPLP SVPA PAPL
Gene ID:25920
Uniprot:Q8WX92
Cellular Location:Nucleus
Calculated MW:65kDa
Observed MW:66kDa
Synonyms:NELFB, COBRA1, NELF-B
Background:
UniProt Protein Function:COBRA1: a subunit of the multisubunit transcription elongation factor NELF (negative elongation factor). May be involved in transcriptional pausing of RNA polymerase II (RNAPII). Interacts with the first BRCT repeat of BRCA1.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleoplasm; cytoplasm

Molecular Function:protein binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription, DNA-dependent

NCBI Summary:NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]
UniProt Code:Q8WX92
NCBI GenInfo Identifier:38372378
NCBI Gene ID:25920
NCBI Accession:Q8WX92.1
UniProt Related Accession:Q8WX92
Molecular Weight:65kDa
NCBI Full Name:Negative elongation factor B
NCBI Synonym Full Names:negative elongation factor complex member B
NCBI Official Symbol:NELFB  
NCBI Official Synonym Symbols:COBRA1; NELF-B  
NCBI Protein Information:negative elongation factor B
UniProt Protein Name:Negative elongation factor B
UniProt Synonym Protein Names:Cofactor of BRCA1
UniProt Gene Name:NELFB  
UniProt Entry Name:NELFB_HUMAN
Anti-NELFB Antibody (CAB12023)Western blot analysis of extracts of various cell lines, using NELFB antibody (CAB12023) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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