Description
| Antibody Name: | Anti-NEK7 Antibody (CAB21088) | 
| Antibody SKU: | CAB21088 | 
| Antibody Size: | 50µL, 100µL | 
| Application: | Western blotting | 
| Reactivity: | Human, Mouse, Rat | 
| Host Species: | Rabbit | 
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 257-301 of human NEK7 (NP_598001.1). | 
| Application: | Western blotting | 
| Recommended Dilution: | WB 1:500 - 1:2000 | 
| Reactivity: | Human, Mouse, Rat | 
| Positive Samples: | Jurkat, HepG2, Mouse lung, Rat liver | 
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 257-301 of human NEK7 (NP_598001.1). | 
| Purification Method: | Affinity purification | 
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.  | 
| Isotype: | IgG | 
| Sequence: | PLPS DHYS EELR QLVN MCIN PDPE KRPD VTYV YDVA KRMH ACTA S | 
| Cellular Location: | |
| Calculated MW: | 35kDa | 
| Observed MW: | 32KDa | 
| Synonyms: | |
| Background: | NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.supplied by OMIM, Jul 2002 | 
![]()  | Western blot analysis of extracts of various cell lines, using NEK7 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. | 
![]()  | Western blot analysis of extracts of various cell lines, using NEK7 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. | 
            
