Cell Cycle Antibodies 1

Anti-NEK4 Antibody (CAB16433)

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SKU:
CAB16433
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-NEK4 Antibody
Antibody SKU:CAB16433
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK4 (NP_003148.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK4 (NP_003148.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPLA AYCY LRVV GKGS YGEV TLVK HRRD GKQY VIKK LNLR NASS RERR AAEQ EAQL LSQL KHPN IVTY KESW EGGD GLLY IVMG FCEG GDLY RKLK EQKG QLLP ENQV VEWF VQIA MALQ YLHE KHIL HRDL KTQN VFLT RTNI IKVG DLGI ARVL ENHC DMAS TLIG TPYY MSPE LFSN KPYN YKSD VWAL GCCV YEMA TLKH AFNA KDMN SLVY RIIE GKLP PMPR DYSP ELAE LIRT MLSK RPEE RPSV RSIL RQPY IKRQ ISFF LEAT KIKT SKNN IKNG DSQS KPFA TVVS GEAE
Gene ID:6787
Uniprot:P51957
Cellular Location:Cell projection, Cytoplasm, cilium
Calculated MW:84kDa/88kDa/94kDa
Observed MW:95kDa
Synonyms:NEK4, NRK2, STK2, pp12301
Background:The protein encoded by this gene is a serine/threonine protein kinase required for normal entry into replicative senescence. The encoded protein also is involved in cell cycle arrest in response to double-stranded DNA damage. Finally, this protein plays a role in maintaining cilium integrity, and defects in this gene have been associated with ciliopathies.
UniProt Protein Function:NEK4: a protein kinase of the NEK family. Highly conserved between the mouse and human and expressed at high levels in the testis.
UniProt Protein Details:

Protein type:Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; NEK family

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: cytoplasm; cilium

Molecular Function:protein serine/threonine kinase activity; manganese ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: mitosis; cell division; mitotic cell cycle; protein amino acid phosphorylation; response to DNA damage stimulus

UniProt Code:P51957
NCBI GenInfo Identifier:116283241
NCBI Gene ID:6787
NCBI Accession:
UniProt Related Accession:P51957,Q05DF6
Molecular Weight:50,822 Da
NCBI Full Name:NEK4 protein
NCBI Synonym Full Names:NIMA (never in mitosis gene a)-related kinase 4
NCBI Official Symbol:NEK4  
NCBI Official Synonym Symbols:NRK2; STK2; pp12301; MGC33171  
NCBI Protein Information:serine/threonine-protein kinase Nek4; OTTHUMP00000213580; OTTHUMP00000213581; OTTHUMP00000213582; serine/threonine kinase 2; nimA-related protein kinase 4; serine/threonine protein kinase-2; serine/threonine-protein kinase 2; never in mitosis A-related kinase 4; serine/threonine-protein kinase NRK2
UniProt Protein Name:NEK4 protein
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:NEK4  
UniProt Entry Name:Q05DF6_HUMAN
Anti-NEK4 Antibody (CAB16433)Western blot analysis of extracts of various cell lines, using NEK4 antibody (CAB16433) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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