Antibody Name:	Anti-NEK3 Antibody
Antibody SKU:	CAB6665
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 267-506 of human NEK3 (NP_689933.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HL-60, THP-1, HepG2, HeLa, Mouse kidney, Rat kidney, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 267-506 of human NEK3 (NP_689933.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PEII MEYG EEVL EEIK NSKH NTPR KKTN PSRI RIAL GNEA STVQ EEEQ DRKG SHTD LESI NENL VESA LRRV NREE KGNK SVHL RKAS SPNL HRRQ WEKN VPNT ALTA LENA SILT SSLT AEDD RGGS VIKY SKNT TRKQ WLKE TPDT LLNI LKNA DLSL AFQT YTIY RPGS EGFL KGPL SEET EASD SVDG GHDS VILD PERL EPGL DEED TDFE EEDD NPDW VSEL KKRA GWQG LCDR
Gene ID:	4752
Uniprot:	P51956
Cellular Location:	Cell projection, Cytoplasm, axon
Calculated MW:	55kDa/57kDa
Observed MW:	55kDa

Synonyms:

NEK3, HSPK36

Background:

This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	NEK3: a protein kinase of the NEK family. May play a role in mitotic regulation.
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, Other; Kinase, protein; Other group; NEK family Chromosomal Location of Human Ortholog: 13q14.13 Cellular Component: axon; cytoplasm; nucleus Molecular Function:protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding Biological Process: mitosis; cell division; protein amino acid phosphorylation
NCBI Summary:	This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]
UniProt Code:	P51956
NCBI GenInfo Identifier:	20178297
NCBI Gene ID:	4752
NCBI Accession:	P51956.2
UniProt Secondary Accession:	P51956,Q5TAP2, Q8J023, Q8WUN5, A8K2J4,
UniProt Related Accession:	P51956
Molecular Weight:	506
NCBI Full Name:	Serine/threonine-protein kinase Nek3
NCBI Synonym Full Names:	NIMA-related kinase 3
NCBI Official Symbol:	NEK3
NCBI Official Synonym Symbols:	HSPK36
NCBI Protein Information:	serine/threonine-protein kinase Nek3; HSPK 36; glycogen synthase A kinase; hydroxyalkyl-protein kinase; nimA-related protein kinase 3; phosphorylase B kinase kinase; never in mitosis A-related kinase 3; NIMA (never in mitosis gene a)-related kinase 3
UniProt Protein Name:	Serine/threonine-protein kinase Nek3
UniProt Synonym Protein Names:	HSPK 36; Never in mitosis A-related kinase 3; NimA-related protein kinase 3
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	NEK3
UniProt Entry Name:	NEK3_HUMAN

	Western blot analysis of extracts of various cell lines, using NEK3 antibody (CAB6665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of HeLa cells using NEK3 antibody (CAB6665). Blue: DAPI for nuclear staining.