Cell Cycle Antibodies 1
Anti-NEK2 Antibody (CAB5355)
- SKU:
- CAB5355
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-NEK2 Antibody |
| Antibody SKU: | CAB5355 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human NEK2 (NP_001191112.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse testis, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human NEK2 (NP_001191112.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPSR AEDY EVLY TIGT GSYG RCQK IRRK SDGK ILVW KELD YGSM TEAE KQML VSEV NLLR ELKH PNIV RYYD RIID RTNT TLYI VMEY CEGG DLAS VITK GTKE RQYL DEEF VLRV MTQL TLAL KECH RRSD GGH |
| Gene ID: | 4751 |
| Uniprot: | P51955 |
| Cellular Location: | Chromosome, Cytoplasm, Nucleus, centromere, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, nucleolus, spindle pole |
| Calculated MW: | 37kDa/44kDa/50kDa/51kDa |
| Observed MW: | 52kDa |
| Synonyms: | NEK2, HsPK21, NEK2A, NLK1, PPP1R111, RP67 |
| Background: | This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. |
| UniProt Protein Function: | NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Nucleolus; Protein kinase, Other; EC 2.7.11.1; Other group; NEK family Chromosomal Location of Human Ortholog: 1q32.3 Cellular Component: kinetochore; spindle pole; microtubule; centrosome; intercellular bridge; protein complex; condensed nuclear chromosome; cytoplasm; nucleolus; midbody; nucleus; cytosol Molecular Function:protein serine/threonine kinase activity; protein binding; metal ion binding; protein phosphatase binding; ATP binding; protein kinase activity Biological Process: mitosis; blastocyst development; regulation of attachment of spindle microtubules to kinetochore; mitotic sister chromatid segregation; protein amino acid autophosphorylation; regulation of mitotic centrosome separation; organelle organization and biogenesis; centrosome separation; spindle assembly; protein amino acid phosphorylation; negative regulation of DNA binding; chromosome segregation; meiotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; regulation of mitosis; mitotic cell cycle; G2/M transition of mitotic cell cycle Disease: Retinitis Pigmentosa 67 |
| NCBI Summary: | This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011] |
| UniProt Code: | P51955 |
| NCBI GenInfo Identifier: | 1709252 |
| NCBI Gene ID: | 4751 |
| NCBI Accession: | P51955.1 |
| UniProt Secondary Accession: | P51955,Q53FD6, Q5I1Z9, Q5VXZ1, Q6NZX8, Q7Z634, Q86XH2 Q96QN9, |
| UniProt Related Accession: | P51955 |
| Molecular Weight: | 445 |
| NCBI Full Name: | Serine/threonine-protein kinase Nek2 |
| NCBI Synonym Full Names: | NIMA-related kinase 2 |
| NCBI Official Symbol: | NEK2 |
| NCBI Official Synonym Symbols: | NLK1; RP67; NEK2A; HsPK21; PPP1R111 |
| NCBI Protein Information: | serine/threonine-protein kinase Nek2; nimA-like protein kinase 1; nimA-related protein kinase 2; protein phosphatase 1, regulatory subunit 111; NIMA (never in mitosis gene a)-related kinase 2 |
| UniProt Protein Name: | Serine/threonine-protein kinase Nek2 |
| UniProt Synonym Protein Names: | HSPK 21; Never in mitosis A-related kinase 2; NimA-related protein kinase 2; NimA-like protein kinase 1 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | NEK2 |
| UniProt Entry Name: | NEK2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NEK2 antibody (CAB5355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human esophagus using NEK2 Antibody (CAB5355) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using NEK2 Antibody (CAB5355) at dilution of 1:100 (40x lens). |
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