Antibody Name:	Anti-NEDD9 Antibody
Antibody SKU:	CAB2521
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-174 of human NEDD9 (NP_892011.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-174 of human NEDD9 (NP_892011.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VKLL IGPM QETA SSHE QPAS GLMQ QTFG QQKL YQVP NPQA APRD TIYQ VPPS YQNQ GIYQ VPTG HGTQ EQEV YQVP PSVQ RSIG GTSG PHVG KKVF QRDG QVSY FLVR ASKQ TSL
Gene ID:	4739
Uniprot:	Q14511
Cellular Location:	Cell junction, Cell projection, Cytoplasm, Golgi apparatus, Nucleus, cell cortex, cytoskeleton, focal adhesion, lamellipodium, spindle
Calculated MW:	19kDa/92kDa
Observed MW:	115kDa

Synonyms:

NEDD9, CAS-L, CAS2, CASL, CASS2, HEF1

Background:

The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	Cas-L: a widely expressed docking protein which plays a central coordinating role for tyrosine-kinase-based signaling in cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. Phosphorylated following integrin beta-1, antigen receptor, or C1a calcitonin receptor signaling. Transformation of fibroblasts with v-ABL results in an increase in its tyrosine phosphorylation. Phosphorylated by focal adhesion kinase. Highly expressed in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 6p24.2 Cellular Component: spindle pole; Golgi apparatus; focal adhesion; lamellipodium; cytoplasm; spindle; cell cortex; nucleus Molecular Function:protein binding Biological Process: integrin-mediated signaling pathway; mitosis; actin filament bundle formation; cell division; regulation of growth; cytoskeleton organization and biogenesis; signal transduction; cell adhesion
NCBI Summary:	The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
UniProt Code:	Q14511
NCBI GenInfo Identifier:	8134360
NCBI Gene ID:	4739
NCBI Accession:	Q14511.1
UniProt Related Accession:	Q14511
Molecular Weight:
NCBI Full Name:	Enhancer of filamentation 1
NCBI Synonym Full Names:	neural precursor cell expressed, developmentally down-regulated 9
NCBI Official Symbol:	NEDD9
NCBI Official Synonym Symbols:	CAS2; CASL; HEF1; CAS-L; CASS2
NCBI Protein Information:	enhancer of filamentation 1
UniProt Protein Name:	Enhancer of filamentation 1
UniProt Synonym Protein Names:	CRK-associated substrate-related protein; CAS-L; CasL; Cas scaffolding protein family member 2; Neural precursor cell expressed developmentally down-regulated protein 9; NEDD-9; Renal carcinoma antigen NY-REN-12; p105Enhancer of filamentation 1 p55
UniProt Gene Name:	NEDD9
UniProt Entry Name:	CASL_HUMAN

	Western blot analysis of extracts of HeLa cells, using NEDD9 antibody (CAB2521) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat kidney using NEDD9 antibody (CAB2521) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of U2OS cells using NEDD9 antibody (CAB2521). Blue: DAPI for nuclear staining.