Signal Transduction Antibodies 1
Anti-NEDD8 Antibody (CAB1163)
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- SKU:
- CAB1163
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-NEDD8 Antibody |
| Antibody SKU: | CAB1163 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human NEDD8 (NP_006147.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse kidney, Rat kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human NEDD8 (NP_006147.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLIK VKTL TGKE IEID IEPT DKVE RIKE RVEE KEGI PPQQ QRLI YSGK QMND EKTA ADYK ILGG SVLH LVLA LRGG GGLR Q |
| Gene ID: | 4738 |
| Uniprot: | Q15843 |
| Cellular Location: | Nucleus |
| Calculated MW: | 9kDa |
| Observed MW: | 12kDa |
| Synonyms: | NEDD8, NEDD-8 |
| Background: |
| UniProt Protein Function: | NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain. |
| UniProt Protein Details: | Protein type:Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 14q12 Cellular Component: cytosol; nucleus Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; protein localization; anatomical structure morphogenesis; protein neddylation; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance |
| UniProt Code: | Q15843 |
| NCBI GenInfo Identifier: | 2833270 |
| NCBI Gene ID: | 4738 |
| NCBI Accession: | Q15843.1 |
| UniProt Secondary Accession: | Q15843,Q3SXN8, Q6LES6, |
| UniProt Related Accession: | Q15843 |
| Molecular Weight: | 81 |
| NCBI Full Name: | NEDD8 |
| NCBI Synonym Full Names: | neural precursor cell expressed, developmentally down-regulated 8 |
| NCBI Official Symbol: | NEDD8 |
| NCBI Official Synonym Symbols: | NEDD-8 |
| NCBI Protein Information: | NEDD8; neddylin; ubiquitin-like protein Nedd8 |
| UniProt Protein Name: | NEDD8 |
| UniProt Synonym Protein Names: | Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8 |
| Protein Family: | NEDD8 |
| UniProt Gene Name: | NEDD8 |
| UniProt Entry Name: | NEDD8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NEDD8 antibody (CAB1163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded human esophagus using NEDD8 antibody (CAB1163) at dilution of 1:100 (40x lens). |
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