Cell Cycle Antibodies 1

Anti-NEDD1 Antibody (CAB5128)

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SKU:
CAB5128
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-NEDD1 Antibody
Antibody SKU:CAB5128
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 411-660 of human NEDD1 (NP_690869.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, Jurkat, mouse brain, rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 411-660 of human NEDD1 (NP_690869.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SPIR DDAV VNKG SDES IGKG DGFD FLPQ LNSV FPPR KNPV TSST SVLH SSPL NVFM GSPG KEEN ENRD LTAE SKKI YMGK QESK DSFK QLAK LVTS GAES GNLN TSPS SNQT RNSE KFEK PENE IEAQ LICE PPIN GSST PNPK IASS VTAG VASS LSEK IADS IGNN RQNA PLTS IQIR FIQN MIQE TLDD FREA CHRD IVNL QVEM IKQF HMQL NEMH SLLE RYSV NEGL VAEI ERLR EENK RLRA HF
Gene ID:121441
Uniprot:Q8NHV4
Cellular Location:Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:62kDa/71kDa/72kDa
Observed MW:78kDa
Synonyms:NEDD1, GCP-WD, TUBGCP7
Background:
UniProt Protein Function:NEDD1: Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 12q23.1

Cellular Component: centriole; nucleoplasm; spindle pole; centrosome; pericentriolar material; apical part of cell; nucleolus; plasma membrane; cytosol

Biological Process: mitosis; cell division; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle

UniProt Code:Q8NHV4
NCBI GenInfo Identifier:74762597
NCBI Gene ID:121441
NCBI Accession:Q8NHV4.1
UniProt Secondary Accession:Q8NHV4,Q8NA30, B0AZN0, B4E145, G3V3F1,
UniProt Related Accession:Q8NHV4
Molecular Weight:660
NCBI Full Name:Protein NEDD1
NCBI Synonym Full Names:neural precursor cell expressed, developmentally down-regulated 1
NCBI Official Symbol:NEDD1  
NCBI Official Synonym Symbols:GCP-WD; TUBGCP7  
NCBI Protein Information:protein NEDD1; NEDD-1; neural precursor cell expressed developmentally down-regulated protein 1
UniProt Protein Name:Protein NEDD1
UniProt Synonym Protein Names:Neural precursor cell expressed developmentally down-regulated protein 1; NEDD-1
Protein Family:Protein
UniProt Gene Name:NEDD1  
UniProt Entry Name:NEDD1_HUMAN
Anti-NEDD1 Antibody (CAB5128)Western blot analysis of extracts of various cell lines, using NEDD1 antibody (CAB5128) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-NEDD1 Antibody (CAB5128)Immunofluorescence analysis of L929 cells using NEDD1 antibody (CAB5128) at dilution of 1:100. Blue: DAPI for nuclear staining.
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