Cell Biology Antibodies 4
Anti-NECAP2 Antibody (CAB14346)
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- SKU:
- CAB14346
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NECAP2 Antibody |
| Antibody SKU: | CAB14346 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 194-263 of human NECAP2 (NP_060560.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse kidney, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 194-263 of human NECAP2 (NP_060560.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GKTS TLIP PPGE QLAV GGSL VQPA VAPS SGGA PVPW PQPN PATA DIWG DFTK STGS TSSQ TQPG TGWV QF |
| Gene ID: | 55707 |
| Uniprot: | Q9NVZ3 |
| Cellular Location: | Cell membrane, Cytoplasmic vesicle, clathrin-coated vesicle membrane |
| Calculated MW: | 19kDa/25kDa/28kDa/29kDa |
| Observed MW: | 28kDa |
| Synonyms: | NECAP2 |
| Background: | This gene likely encodes a member of the adaptin-ear-binding coat-associated protein family. Studies of a similar protein in rat suggest a role in clathrin-mediated endocytosis. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | NECAP2: Involved in endocytosis. Belongs to the NECAP family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 1p36.13 Cellular Component: clathrin vesicle coat; plasma membrane; coated pit; intracellular Biological Process: protein transport; endocytosis |
| NCBI Summary: | This gene likely encodes a member of the adaptin-ear-binding coat-associated protein family. Studies of a similar protein in rat suggest a role in clathrin-mediated endocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009] |
| UniProt Code: | Q9NVZ3 |
| NCBI GenInfo Identifier: | 8922416 |
| NCBI Gene ID: | 55707 |
| NCBI Accession: | NP_060560.1 |
| UniProt Secondary Accession: | Q9NVZ3,Q5VSU4, Q5VSU5, Q9H7L1, Q9H8L1, B4DY19, E9PGQ8 |
| UniProt Related Accession: | Q9NVZ3 |
| Molecular Weight: | 25,267 Da |
| NCBI Full Name: | adaptin ear-binding coat-associated protein 2 isoform 1 |
| NCBI Synonym Full Names: | NECAP endocytosis associated 2 |
| NCBI Official Symbol: | NECAP2 |
| NCBI Protein Information: | adaptin ear-binding coat-associated protein 2; NECAP-2; NECAP endocytosis-associated protein 2; adaptin-ear-binding coat-associated protein 2 |
| UniProt Protein Name: | Adaptin ear-binding coat-associated protein 2 |
| UniProt Synonym Protein Names: | NECAP endocytosis-associated protein 2; NECAP-2 |
| Protein Family: | Adaptin ear-binding coat-associated protein |
| UniProt Gene Name: | NECAP2 |
| UniProt Entry Name: | NECP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NECAP2 Rabbit pAb (CAB14346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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