Antibody Name:	Anti-NDUFV2 Antibody
Antibody SKU:	CAB7442
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human NDUFV2 (NP_066552.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HT-29, PC-12, Mouse liver, Mouse brain, Rat liver, HL-60, NCI-H460, SKOV3, A-549, Mouse kidney, Mouse heart, Mouse testis, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human NDUFV2 (NP_066552.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFFS AALR ARAA GLTA HWGR HVRN LHKT VMQN GAGG ALFV HRDT PENN PDTP FDFT PENY KRIE AIVK NYPE GHKA AAVL PVLD LAQR QNGW LPIS AMNK VAEV LQVP PMRV YEVA TFYT MYNR KPVG KYHI QVCT TTPC MLRN SDSI LEAI QKKL GIKV GETT PDKL FTLI EVEC LGAC VNAP MVQI NDNY YEDL TAKD IEEI IDEL KAGK IPKP GPRS GRFS CEPA GGLT SLTE PPKG PGFG VQAG L
Gene ID:	4729
Uniprot:	P19404
Cellular Location:	Mitochondrion inner membrane
Calculated MW:	27kDa
Observed MW:	27kDa

Synonyms:

NDUFV2, CI-24k

Background:

The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes the 24 kDa subunit of complex I, and is involved in electron transfer. Mutations in this gene are implicated in Parkinson's disease, bipolar disorder, schizophrenia, and have been found in one case of early onset hypertrophic cardiomyopathy and encephalopathy. A non-transcribed pseudogene of this locus is found on chromosome 19.

UniProt Protein Function:	NDUFV2: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I 24 kDa subunit family.
UniProt Protein Details:	Protein type:Oxidoreductase; Mitochondrial; EC 1.6.99.3; EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 18p11.22 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex I Molecular Function:2 iron, 2 sulfur cluster binding; electron carrier activity; NADH dehydrogenase (ubiquinone) activity; metal ion binding Biological Process: cardiac muscle development; cellular metabolic process; nervous system development; mitochondrial electron transport, NADH to ubiquinone Disease: Mitochondrial Complex I Deficiency
NCBI Summary:	The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes the 24 kDa subunit of complex I, and is involved in electron transfer. Mutations in this gene are implicated in Parkinson's disease, bipolar disorder, schizophrenia, and have been found in one case of early onset hypertrophic cardiomyopathy and encephalopathy. A non-transcribed pseudogene of this locus is found on chromosome 19. [provided by RefSeq, Oct 2009]
UniProt Code:	P19404
NCBI GenInfo Identifier:	20455499
NCBI Gene ID:	4729
NCBI Accession:	P19404.2
UniProt Secondary Accession:	P19404,Q9BV41,
UniProt Related Accession:	P19404
Molecular Weight:	249
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
NCBI Official Symbol:	NDUFV2
NCBI Official Synonym Symbols:	CI-24k
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; complex I 24kDa subunit; NADH-ubiquinone oxidoreductase 24 kDa subunit; NADH-ubiquinone oxidoreductase flavoprotein 2; complex I, mitochondrial respitory chain, 24 kD subunit; NADH dehydrogenase ubiquinone flavoprotein 2, mitochondrial; nuclear-encoded mitochondrial NADH-ubiquinone reductase 24Kd subunit
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
UniProt Synonym Protein Names:	NADH-ubiquinone oxidoreductase 24 kDa subunit
Protein Family:	NADH dehydrogenase [ubiquinone] flavoprotein
UniProt Gene Name:	NDUFV2
UniProt Entry Name:	NDUV2_HUMAN

	Western blot analysis of extracts of various cell lines, using NDUFV2 antibody (CAB7442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat kidney using NDUFV2 antibody (CAB7442) at dilution of 1:100 (40x lens).