Antibody Name:	Anti-NDUFB3 Antibody
Antibody SKU:	CAB14378
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human NDUFB3 (NP_002482.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse heart, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human NDUFB3 (NP_002482.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAHE HGHE HGHH KMEL PDYR QWKI EGTP LETI QKKL AAKG LRDP WGRN EAWR YMGG FAKS VSFS DVFF KGFK WGFA AFVV AVGA EYYL ESLN KDKK HH
Gene ID:	4709
Uniprot:	O43676
Cellular Location:	Matrix side, Mitochondrion inner membrane, Single-pass membrane protein
Calculated MW:	11kDa
Observed MW:	11kDa

Synonyms:

NDUFB3, B12, CI-B12

Background:

This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which is the first enzyme in the electron transport chain of mitochondria. This protein localizes to the inner membrane of the mitochondrion as a single-pass membrane protein. Mutations in this gene contribute to mitochondrial complex 1 deficiency. Alternative splicing results in multiple transcript variants encoding the same protein. Humans have multiple pseudogenes of this gene.

UniProt Protein Function:	NDUFB3: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB3 subunit family.
UniProt Protein Details:	Protein type:Membrane protein, integral; EC 1.6.99.3; Energy Metabolism - oxidative phosphorylation; Mitochondrial; EC 1.6.5.3; Oxidoreductase Chromosomal Location of Human Ortholog: 2q31.3 Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly Disease: Mitochondrial Complex I Deficiency
NCBI Summary:	This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which is the first enzyme in the electron transport chain of mitochondria. This protein localizes to the inner membrane of the mitochondrion as a single-pass membrane protein. Mutations in this gene contribute to mitochondrial complex 1 deficiency. Alternative splicing results in multiple transcript variants encoding the same protein. Humans have multiple pseudogenes of this gene. [provided by RefSeq, Mar 2012]
UniProt Code:	O43676
NCBI GenInfo Identifier:	6093472
NCBI Gene ID:	4709
NCBI Accession:	O43676.3
UniProt Secondary Accession:	O43676,Q6IB80,
UniProt Related Accession:	O43676
Molecular Weight:	11,402 Da
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH:ubiquinone oxidoreductase subunit B3
NCBI Official Symbol:	NDUFB3
NCBI Official Synonym Symbols:	B12; CI-B12
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
UniProt Synonym Protein Names:	Complex I-B12; CI-B12; NADH-ubiquinone oxidoreductase B12 subunit
Protein Family:	NADH dehydrogenase
UniProt Gene Name:	NDUFB3
UniProt Entry Name:	NDUB3_HUMAN

	Western blot analysis of extracts of various cell lines, using NDUFB3 antibody (CAB14378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of L929 cells using NDUFB3 Polyclonal Antibody (CAB14378) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.