ChIP Antibodies

Anti-NDUFAF5 Antibody (CAB7135)

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SKU:
CAB7135
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NDUFAF5 Antibody
Antibody SKU:CAB7135
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 196-345 of human NDUFAF5 (NP_077025.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, SGC7901
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 196-345 of human NDUFAF5 (NP_077025.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ELRC SLQL AETE REGG FSPH ISPF TAVN DLGH LLGR AGFN TLTV DTDE IQVN YPGM FELM EDLQ GMGE SNCA WNRK ALLH RDTM LAAA AVYR EMYR NEDG SVPA TYQI YYMI GWKY HESQ ARPA ERGS ATVS FGEL GKIN NLMP PGKK SQ
Gene ID:79133
Uniprot:Q5TEU4
Cellular Location:Mitochondrion inner membrane
Calculated MW:36kDa/38kDa
Observed MW:39kDa
Synonyms:NDUFAF5, C20orf7, bA526K24.2, dJ842G6.1
Background:The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:NDUFAF5: Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I, MT-ND1) at early stages. May have methyltransferase activity. Defects in NDUFAF5 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Defects in NDUFAF5 are a cause of Leigh syndrome (LS). An early-onset progressive neurodegenerative disorder characterized by the presence of focal, bilateral lesions in one or more areas of the central nervous system including the brainstem, thalamus, basal ganglia, cerebellum and spinal cord. Clinical features depend on which areas of the central nervous system are involved and include subacute onset of psychomotor retardation, hypotonia, ataxia, weakness, vision loss, eye movement abnormalities, seizures, and dysphagia. Belongs to the methyltransferase superfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.1.1.-; Mitochondrial; Methyltransferase

Chromosomal Location of Human Ortholog: 20p12.1

Molecular Function:methyltransferase activity

Biological Process: methylation; mitochondrial respiratory chain complex I assembly

Disease: Mitochondrial Complex I Deficiency

NCBI Summary:The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:Q5TEU4
NCBI GenInfo Identifier:74762247
NCBI Gene ID:79133
NCBI Accession:Q5TEU4.1
UniProt Secondary Accession:Q5TEU4,Q6GPH3, Q9H6F4, A8K166,
UniProt Related Accession:Q5TEU4
Molecular Weight:345
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH dehydrogenase (ubiquinone) complex I, assembly factor 5
NCBI Official Symbol:NDUFAF5  
NCBI Official Synonym Symbols:C20orf7; dJ842G6.1; bA526K24.2  
NCBI Protein Information:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5; probable methyltransferase C20orf7, mitochondrial
UniProt Protein Name:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5
UniProt Synonym Protein Names:Probable methyltransferase C20orf7, mitochondrial (EC:2.1.1.-)
Protein Family:Arginine-hydroxylase
UniProt Gene Name:NDUFAF5  
UniProt Entry Name:NDUF5_HUMAN
Anti-NDUFAF5 Antibody (CAB7135)Western blot analysis of extracts of various cell lines, using NDUFAF5 antibody (CAB7135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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