Name: Anti-NDUFAF1 Antibody (CAB15835)
Brand: Signal Transduction Antibodies 2
SKU: CAB15835
Availability: OutOfStock

Antibody Name:	Anti-NDUFAF1 Antibody
Antibody SKU:	CAB15835
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human NDUFAF1 (NP_057097.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T, A-549, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human NDUFAF1 (NP_057097.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MALV HKLL RGTY FLRK FSKP TSAL YPFL GIRF AEYS SSLQ KPVA SPGK ASSQ RKTE GDLQ GDHQ KEVA LDIT SSEE KPDV SFDK AIRD EAIY HFRL LKDE IVDH WRGP EGHP LHEV LLEQ AKVV WQFR GKED LDKW TVTS DKTI GGRS EVFL KMGK NNQS ALLY GTLS SEAP QDGE STRS GYCA MISR IPRG AFER KMSY DWSQ FNTL YLRV RGDG RPWM VNIK EDTD FFQR TNQM YSYF MFTR GGPY WQEV KIPF SKFF FSNR GRIR DVQH ELPL DKIS SIGF TLAD KVDG PFFL EIDF IGVF TDPA HTEE FAYE NSPE LNPR LFK
Gene ID:	51103
Uniprot:	Q9Y375
Cellular Location:	Mitochondrion
Calculated MW:	37kDa
Observed MW:	38kDa

Synonyms:

NDUFAF1, CGI-65, CGI65, CIA30

Background:

This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19.

UniProt Protein Function:	NDUFAF1: Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Belongs to the CIA30 family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 15q11.2-q21.3 Cellular Component: cytoplasm; mitochondrial inner membrane Molecular Function:protein binding Biological Process: mitochondrial respiratory chain complex I assembly Disease: Mitochondrial Complex I Deficiency
NCBI Summary:	This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. [provided by RefSeq, Dec 2011]
UniProt Code:	Q9Y375
NCBI GenInfo Identifier:	21542405
NCBI Gene ID:	51103
NCBI Accession:	Q9Y375.2
UniProt Secondary Accession:	Q9Y375,Q9BVZ5,
UniProt Related Accession:	Q9Y375
Molecular Weight:	37,764 Da
NCBI Full Name:	Complex I intermediate-associated protein 30, mitochondrial
NCBI Synonym Full Names:	NADH:ubiquinone oxidoreductase complex assembly factor 1
NCBI Official Symbol:	NDUFAF1
NCBI Official Synonym Symbols:	CGI65; CIA30; CGI-65
NCBI Protein Information:	complex I intermediate-associated protein 30, mitochondrial
UniProt Protein Name:	Complex I intermediate-associated protein 30, mitochondrial
UniProt Synonym Protein Names:	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1
UniProt Gene Name:	NDUFAF1
UniProt Entry Name:	CIA30_HUMAN

Western blot analysis of extracts of various cell lines, using NDUFAF1 antibody (CAB15835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 35s.

Antibodies

NDUFAF1 Antibody (PACO10782)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)