KO Validated Antibodies 1

Anti-NDUFA7 Antibody (CAB8441)[KO Validated]

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SKU:
CAB8441
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NDUFA7 Antibody [KO Validated]
Antibody SKU:CAB8441
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, HepG2, MCF-7, Mouse kidney, Mouse brain, Mouse liver, Mouse heart, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MASA TRLI QRLR NWAS GHDL QGKL QLRY QEIS KRTQ PPPK LPVG PSHK LSNN YYCT RDGR RESV PPSI IMSS QKAL VSGK PAES SAVA ATEK KAVT PAPP IKRW ELSS DQPY L
Gene ID:4701
Uniprot:O95182
Cellular Location:Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:12kDa
Observed MW:13kDa
Synonyms:NDUFA7, B14.5a, CI-B14.5a
Background:This gene encodes a subunit of NADH:ubiquinone oxidoreductase (complex I), which is a multiprotein complex located in the inner mitochondrial membrane. Complex I functions in the transfer of electrons from NADH to the respiratory chain.
UniProt Protein Function:NDUFA7: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA7 subunit family.
UniProt Protein Details:

Protein type:Oxidoreductase; Mitochondrial; EC 1.6.99.3; EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I; mitochondrial ribosome; mitochondrion

Molecular Function:structural constituent of ribosome

Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; mitochondrial translation

NCBI Summary:This gene encodes a subunit of NADH:ubiquinone oxidoreductase (complex I), which is a multiprotein complex located in the inner mitochondrial membrane. Complex I functions in the transfer of electrons from NADH to the respiratory chain. [provided by RefSeq, Mar 2011]
UniProt Code:O95182
NCBI GenInfo Identifier:6093463
NCBI Gene ID:4701
NCBI Accession:O95182.3
UniProt Related Accession:O95182
Molecular Weight:12,551 Da
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH:ubiquinone oxidoreductase subunit A7
NCBI Official Symbol:NDUFA7  
NCBI Official Synonym Symbols:B14.5a; CI-B14.5a  
NCBI Protein Information:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
UniProt Protein Name:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
UniProt Synonym Protein Names:Complex I-B14.5a; CI-B14.5a; NADH-ubiquinone oxidoreductase subunit B14.5a
Protein Family:NADH dehydrogenase
UniProt Gene Name:NDUFA7  
UniProt Entry Name:NDUA7_HUMAN
Anti-NDUFA7 Polyclonal Antibody [KO Validated] (CAB8441)Western blot analysis of extracts of various cell lines, using NDUFA7 antibody (CAB8441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-NDUFA7 Polyclonal Antibody [KO Validated] (CAB8441)Western blot analysis of extracts from normal (control) and NDUFA7 knockout (KO) 293T cells, using NDUFA7 antibody (CAB8441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-NDUFA7 Polyclonal Antibody [KO Validated] (CAB8441)Immunohistochemistry of paraffin-embedded human liver damage using NDUFA7 antibody (CAB8441) at dilution of 1:100 (40x lens).
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