Cell Biology Antibodies 12

Anti-NDUFA6 Antibody (CAB9831)

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SKU:
CAB9831
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NDUFA6 Antibody
Antibody SKU:CAB9831
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 35-154 of human NDUFA6 (NP_002481.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Raji, HepG2, 22Rv1, Mouse kidney, Mouse heart, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 35-154 of human NDUFA6 (NP_002481.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ATST ASTF VKPI FSRD MNEA KRRV RELY RAWY REVP NTVH QFQL DITV KMGR DKVR EMFM KNAH VTDP RVVD LLVI KGKI ELEE TIKV WKQR THVM RFFH ETEA PRPK DFLS KFYV GHDP
Gene ID:4700
Uniprot:P56556
Cellular Location:Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:17kDa
Observed MW:13kDa
Synonyms:NDUFA6, B14, CI-B14, LYRM6, NADHB14
Background:
UniProt Protein Function:NDUFA6: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I LYR family.
UniProt Protein Details:

Protein type:EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation; Mitochondrial; Oxidoreductase; EC 1.6.99.3

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: mitochondrial inner membrane; mitochondrial membrane; mitochondrial respiratory chain complex I

Molecular Function:NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; response to oxidative stress

UniProt Code:P56556
NCBI GenInfo Identifier:51317370
NCBI Gene ID:4700
NCBI Accession:NP_002481.2
UniProt Secondary Accession:P56556,O43675, Q6FGW0, Q6IBT8, Q6IC39, B2RE54,
UniProt Related Accession:P56556
Molecular Weight:17,871 Da
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH:ubiquinone oxidoreductase subunit A6
NCBI Official Symbol:NDUFA6  
NCBI Official Synonym Symbols:B14; LYRM6; CI-B14; NADHB14  
NCBI Protein Information:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
UniProt Protein Name:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
UniProt Synonym Protein Names:Complex I-B14; CI-B14; LYR motif-containing protein 6; NADH-ubiquinone oxidoreductase B14 subunit
Protein Family:NADH dehydrogenase
UniProt Gene Name:NDUFA6  
UniProt Entry Name:NDUA6_HUMAN
Anti-NDUFA6 Polyclonal Antibody (CAB9831)Western blot analysis of extracts of various cell lines, using NDUFA6 antibody (CAB9831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-NDUFA6 Polyclonal Antibody (CAB9831)Immunohistochemistry of paraffin-embedded mouse lung using NDUFA6 antibody (CAB9831) at dilution of 1:100 (40x lens).
Anti-NDUFA6 Polyclonal Antibody (CAB9831)Immunohistochemistry of paraffin-embedded human stomach using NDUFA6 antibody (CAB9831) at dilution of 1:100 (40x lens).
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