Antibody Name:	NDUFA2 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8136
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human NDUFA2 (NP_002479.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human NDUFA2 (NP_002479.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA AASR GVGA KLGL REIR IHLC QRSP GSQG VRDF IEKR YVEL KKAN PDLP ILIR ECSD VQPK LWAR YAFG QETN VPLN NFSA DQVT RALE NVL
Gene ID:	4695
Uniprot:	O43678
Cellular Location:	Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	8kDa/10kDa
Observed MW:	11kDa

Synonyms:

NDUFA2, B8, CD14, CIB8

Background:

The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	NDUFA2: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
UniProt Protein Details:	Protein type:Mitochondrial; EC 1.6.99.3; Oxidoreductase; EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 5q31.2 Cellular Component: mitochondrial inner membrane; mitochondrial membrane; mitochondrial respiratory chain complex I Molecular Function:NADH dehydrogenase (ubiquinone) activity Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly Disease: Leigh Syndrome
NCBI Summary:	The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Code:	O43678
NCBI GenInfo Identifier:	297206774
NCBI Gene ID:	4695
NCBI Accession:	NP_001171941.1
UniProt Secondary Accession:	O43678,Q6IAY8, D6RJD6,
UniProt Related Accession:	O43678
Molecular Weight:	8,491 Da
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH:ubiquinone oxidoreductase subunit A2
NCBI Official Symbol:	NDUFA2
NCBI Official Synonym Symbols:	B8; CD14; CIB8
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
UniProt Synonym Protein Names:	Complex I-B8; CI-B8; NADH-ubiquinone oxidoreductase B8 subunit
Protein Family:	NADH dehydrogenase
UniProt Gene Name:	NDUFA2
UniProt Entry Name:	NDUA2_HUMAN

	Western blot analysis of extracts of various cell lines, using NDUFA2 antibody (CAB8136) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human esophagus using NDUFA2 antibody (CAB8136) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using NDUFA2 antibody (CAB8136) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH/3T3 cells using NDUFA2 antibody (CAB8136) at dilution of 1:100. Blue: DAPI for nuclear staining.