Antibody Name:	Anti-NDRG4 Antibody
Antibody SKU:	CAB16195
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 140-220 of human NDRG4 (NP_001229765.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 140-220 of human NDRG4 (NP_001229765.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DPNG KGWI DWAA TKLS GLTS TLPD TVLS HLFS QEEL VNNT ELVQ SYRQ QIGN VVNQ ANLQ LFWN MYNS RRDL DINR PGTV P
Gene ID:	65009
Uniprot:	Q9ULP0
Cellular Location:	Cytoplasm, cytosol
Calculated MW:	37kDa/38kDa/39kDa/40kDa/42kDa
Observed MW:	38kDa

Synonyms:

NDRG4, BDM1, SMAP-8, SMAP8

Background:

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.

UniProt Protein Function:	NDRG4: Contributes to the maintenance of intracerebral BDNF levels within the normal range, which is necessary for the preservation of spatial learning and the resistance to neuronal cell death caused by ischemic stress. May enhance growth factor-induced ERK1 and ERK2 phosphorylation, including that induced by PDGF and FGF. May attenuate NGF-promoted ELK1 phosphorylation in a microtubule-dependent manner. Expressed predominantly in brain and heart. In the brain, detected in astrocytes. Isoform 1 and isoform 2 are only expressed in brain. Isoform 3 is expressed in both heart and brain. Up-regulated in glioblastoma multiforme cells. Belongs to the NDRG family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 16q21 Cellular Component: endoplasmic reticulum membrane; mitochondrion; basolateral plasma membrane; cytoplasm; cytosol Biological Process: negative regulation of smooth muscle cell proliferation; response to stress; embryonic heart tube development; cardiac muscle cell proliferation; heart looping; negative regulation of smooth muscle cell migration; cell differentiation; cell growth
NCBI Summary:	This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.[provided by RefSeq, Jun 2011]
UniProt Code:	Q9ULP0
NCBI GenInfo Identifier:	20141614
NCBI Gene ID:	65009
NCBI Accession:	Q9ULP0.2
UniProt Secondary Accession:	Q9ULP0,Q6ZNE7, Q6ZTI7, Q96PL9, Q9GZM1, Q9GZN3, Q9GZX0 B3KNU2, B4DK66, B4DSW5, H7C600,
UniProt Related Accession:	Q9ULP0
Molecular Weight:	352
NCBI Full Name:	Protein NDRG4
NCBI Synonym Full Names:	NDRG family member 4
NCBI Official Symbol:	NDRG4
NCBI Official Synonym Symbols:	BDM1; SMAP8; SMAP-8
NCBI Protein Information:	protein NDRG4; smooth muscle-associated protein 8; brain development-related molecule 1; N-myc downstream-regulated gene 4 protein; vascular smooth muscle cell-associated protein 8
UniProt Protein Name:	Protein NDRG4
UniProt Synonym Protein Names:	Brain development-related molecule 1; N-myc downstream-regulated gene 4 protein; Vascular smooth muscle cell-associated protein 8; SMAP-8
Protein Family:	Protein
UniProt Gene Name:	NDRG4
UniProt Entry Name:	NDRG4_HUMAN

	Western blot analysis of extracts of various cell lines, using NDRG4 Rabbit pAb (CAB16195) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using NDRG4 antibody (CAB16195) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using NDRG4 antibody (CAB16195) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using NDRG4 antibody (CAB16195) at dilution of 1:100. Blue: DAPI for nuclear staining.