Antibody Name:	Anti-NCS1 Antibody
Antibody SKU:	CAB13586
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human NCS1 (NP_055101.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SW620, MCF7, U-251MG, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human NCS1 (NP_055101.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGKS NSKL KPEV VEEL TRKT YFTE KEVQ QWYK GFIK DCPS GQLD AAGF QKIY KQFF PFGD PTKF ATFV FNVF DENK DGRI EFSE FIQA LSVT SRGT LDEK LRWA FKLY DLDN DGYI TRNE MLDI VDAI YQMV GNTV ELPE EENT PEKR VDRI FAMM DKNA DGKL TLQE FQEG SKAD PSIV QALS LYDG LV
Gene ID:	23413
Uniprot:	P62166
Cellular Location:	Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Golgi stack membrane, Lipid-anchor, Membrane, Peripheral membrane protein, perinuclear region, postsynaptic cell membrane, postsynaptic density, synapse
Calculated MW:	19kDa/21kDa
Observed MW:	22kDa

Synonyms:

NCS1, FLUP, FREQ

Background:

This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	FREQ: Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel. Interacts with KCND2. Interacts in a calcium-independent manner with PI4KB. This binding competes with CALN2/CABP7 binding to PI4KB. Interacts with ARF1, ARF3, ARF5 and ARF6. Interacts in a calcium-dependent manner with PICK1 (via AH domain). Interacts with IL1RAPL1. Belongs to the recoverin family.
UniProt Protein Details:	Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 9q34 Cellular Component: cytoplasm; intracellular membrane-bound organelle; plasma membrane Molecular Function:calcium ion binding; protein binding; voltage-gated calcium channel activity
NCBI Summary:	This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P62166
NCBI GenInfo Identifier:	49065666
NCBI Gene ID:	23413
NCBI Accession:	P62166.2
UniProt Secondary Accession:	P62166,P36610, Q9UK26, E9PAY3,
UniProt Related Accession:	P62166
Molecular Weight:	19,728 Da
NCBI Full Name:	Neuronal calcium sensor 1
NCBI Synonym Full Names:	neuronal calcium sensor 1
NCBI Official Symbol:	NCS1
NCBI Official Synonym Symbols:	FLUP; FREQ
NCBI Protein Information:	neuronal calcium sensor 1
UniProt Protein Name:	Neuronal calcium sensor 1
UniProt Synonym Protein Names:	Frequenin homolog; Frequenin-like protein; Frequenin-like ubiquitous protein
Protein Family:	Frequenin
UniProt Gene Name:	NCS1
UniProt Entry Name:	NCS1_HUMAN

	Western blot analysis of extracts of various cell lines, using NCS1 antibody (CAB13586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using NCS1 antibody (CAB13586). Blue: DAPI for nuclear staining.