Cell Biology Antibodies 10

Anti-NCOR1 Antibody (CAB7046)

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SKU:
CAB7046
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NCOR1 Antibody
Antibody SKU:CAB7046
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human NCOR1 (NP_006302.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human NCOR1 (NP_006302.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSSS GYPP NQGA FSTE QSRY PPHS VQYT FPNT RHQQ EFAV PDYR SSHL EVSQ ASQL LQQQ QQQQ LRRR PSLL SEFH PGSD RPQE RRTS YEPF HPGP SPVD HDSL ESKR PRLE QVSD SHFQ RVSA AVLP LVHP LPEG LRAS ADAK KDPA FGGK HEAP SSPI SGQP CGDD QNAS PSKL SKEE LIQS MDRV DREI AKVE QQIL
Gene ID:9611
Uniprot:O75376
Cellular Location:Nucleus
Calculated MW:103kDa/258kDa/270kDa
Observed MW:270KDa
Synonyms:NCOR1, N-CoR, N-CoR1, PPP1R109, TRAC1, hN-CoR
Background:This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.
UniProt Protein Function:N-CoR1: is a protein that regulates the activity of some transcription factors, including nuclear receptors, by promoting histone deacetylation and chromatin condensation, thereby altering the accessibility of various genes to the transcriptional machinery. Is known to play a role genetic programming including that for myogenesis. Is a component of a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid and the retinoic acid receptors in the absence of ligand. Interacts with the catalytic domain of HDAC9. Increased expression correlates with the loss of vitamin D responsiveness in aggressive androgen-independent prostate cancer cells. Its gene tends to be overexpressed in multiple-myeloma cell lines.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: histone deacetylase complex; membrane; nuclear chromatin; nucleoplasm; nucleus; Sin3 complex; spindle microtubule; transcriptional repressor complex

Molecular Function:histone deacetylase binding; ligand-dependent nuclear receptor binding; nuclear hormone receptor binding; protein binding; thyroid hormone receptor binding; transcription corepressor activity

Biological Process: cellular lipid metabolic process; circadian rhythm; negative regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; spindle assembly; transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.[provided by RefSeq, Jun 2010]
UniProt Code:O75376
NCBI GenInfo Identifier:47117817
NCBI Gene ID:9611
NCBI Accession:O75376.2
UniProt Secondary Accession:O75376,Q86YY0, Q9UPV5, Q9UQ18, B3DLF8, E9PGV6,
UniProt Related Accession:O75376
Molecular Weight:
NCBI Full Name:Nuclear receptor corepressor 1
NCBI Synonym Full Names:nuclear receptor corepressor 1
NCBI Official Symbol:NCOR1  
NCBI Official Synonym Symbols:N-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109  
NCBI Protein Information:nuclear receptor corepressor 1
UniProt Protein Name:Nuclear receptor corepressor 1
Protein Family:Nuclear receptor corepressor
UniProt Gene Name:NCOR1  
UniProt Entry Name:NCOR1_HUMAN
Anti-NCOR1 Antibody (CAB7046)Western blot analysis of extracts of Jurkat cells, using NCOR1 antibody (CAB7046) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-NCOR1 Antibody (CAB7046)Immunohistochemistry of paraffin-embedded rat stomach using NCOR1 Rabbit pAb (CAB7046) at dilution of 1:100 (40x lens).
Anti-NCOR1 Antibody (CAB7046)Immunohistochemistry of paraffin-embedded human colon carcinoma using NCOR1 Rabbit pAb (CAB7046) at dilution of 1:100 (40x lens).
Anti-NCOR1 Antibody (CAB7046)Immunohistochemistry of paraffin-embedded mouse testis using NCOR1 Rabbit pAb (CAB7046) at dilution of 1:100 (40x lens).
Anti-NCOR1 Antibody (CAB7046)Immunofluorescence analysis of U2OS cells using NCOR1 antibody (CAB7046). Blue: DAPI for nuclear staining.
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