Epigenetics & Nuclear Signaling Antibodies 1
Anti-NCOA7 Antibody (CAB13799)
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- SKU:
- CAB13799
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-NCOA7 Antibody |
| Antibody SKU: | CAB13799 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NCOA7 (NP_861447.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, MCF7, OVCAR3, HeLa, DU145, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NCOA7 (NP_861447.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDTK EEKK ERKQ SYFA RLKK KKQA KQNA ETAS AVAT RTHT GKED NNTV VLEP DKCN IAVE EEYM TDEK KKRK SNQL KEIR RTEL KRYY SI |
| Gene ID: | 135112 |
| Uniprot: | Q8NI08 |
| Cellular Location: | Nucleus |
| Calculated MW: | 12kDa/25kDa/28kDa/33kDa/92kDa/104kDa/106kDa |
| Observed MW: | 130kDa |
| Synonyms: | NCOA7, ERAP140, ESNA1, NCOA7-AS, Nbla00052, Nbla10993, TLDC4, dJ187J11.3 |
| Background: |
| UniProt Protein Function: | NCOA7: Enhances the transcriptional activities of several nuclear receptors. Involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA. Belongs to the OXR1 family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 6q22.32 Cellular Component: intracellular Molecular Function:ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; protein binding Biological Process: positive regulation of transcription from RNA polymerase II promoter |
| UniProt Code: | Q8NI08 |
| NCBI GenInfo Identifier: | 110287684 |
| NCBI Gene ID: | 135112 |
| NCBI Accession: | Q8NI08.2 |
| UniProt Secondary Accession: | Q8NI08,Q3LID6, Q4G0V1, Q5TF95, Q6IPQ4, Q6NE83, Q86T89 Q8N1W4, B2RNS2, B7Z2C4, B9EH71, G8JL91, |
| UniProt Related Accession: | Q8NI08 |
| Molecular Weight: | 92,472 Da |
| NCBI Full Name: | Nuclear receptor coactivator 7 |
| NCBI Synonym Full Names: | nuclear receptor coactivator 7 |
| NCBI Official Symbol: | NCOA7 |
| NCBI Official Synonym Symbols: | ESNA1; TLDC4; ERAP140; NCOA7-AS; Nbla00052; Nbla10993; dJ187J11.3 |
| NCBI Protein Information: | nuclear receptor coactivator 7 |
| UniProt Protein Name: | Nuclear receptor coactivator 7 |
| UniProt Synonym Protein Names: | 140 kDa estrogen receptor-associated protein; Estrogen nuclear receptor coactivator 1 |
| Protein Family: | Nuclear receptor coactivator |
| UniProt Gene Name: | NCOA7 |
| UniProt Entry Name: | NCOA7_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NCOA7 antibody (CAB13799) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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