Name: Anti-NCOA5 Antibody (CAB3476)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB3476
Availability: OutOfStock

Antibody Name:	Anti-NCOA5 Antibody
Antibody SKU:	CAB3476
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human NCOA5 (NP_066018.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	U-87MG, HeLa, Jurkat, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human NCOA5 (NP_066018.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QALE DVSR GGSP FAIV ITQQ HQIH RSCT VNIM FGTP QEHR NMPQ ADAM VLVA RNYE RYKN ECRE KERE EIAR QAAK MADE AILQ ERER GGPE EGVR GGHP PAIQ SLIN LLAD NRYL TAEE TDKI INYL RERK ERLM RSST D
Gene ID:	57727
Uniprot:	Q9HCD5
Cellular Location:	Nucleus
Calculated MW:	65kDa
Observed MW:	65kDa

Synonyms:

NCOA5, CIA, bA465L10.6

Background:

This gene encodes a coregulator for the alpha and beta estrogen receptors and the orphan nuclear receptor NR1D2. The protein localizes to the nucleus, and is thought to have both coactivator and corepressor functions. Its interaction with nuclear receptors is independent of the AF2 domain on the receptors, which is known to regulate interaction with other coreceptors. Several alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	NCOA5: Nuclear receptor coregulator that can have both coactivator and corepressor functions. Interacts with nuclear receptors for steroids (ESR1 and ESR2) independently of the steroid binding domain (AF-2) of the ESR receptors, and with the orphan nuclear receptor NR1D2. Involved in the coactivation of nuclear steroid receptors (ER) as well as the corepression of MYC in response to 17-beta-estradiol (E2).
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: nucleoplasm; extracellular space; nucleus; actin cytoskeleton Molecular Function:protein binding; chromatin binding Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; glucose homeostasis
NCBI Summary:	This gene encodes a coregulator for the alpha and beta estrogen receptors and the orphan nuclear receptor NR1D2. The protein localizes to the nucleus, and is thought to have both coactivator and corepressor functions. Its interaction with nuclear receptors is independent of the AF2 domain on the receptors, which is known to regulate interaction with other coreceptors. Two alternatively spliced transcript variants for this gene have been described. However, the full length nature of one of the variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9HCD5
NCBI GenInfo Identifier:	28380083
NCBI Gene ID:	57727
NCBI Accession:	Q9HCD5.2
UniProt Secondary Accession:	Q9HCD5,Q6HA99, Q9H1F2, Q9H2T2, Q9H4Y9, B2RTV9, E1P5R0
UniProt Related Accession:	Q9HCD5
Molecular Weight:	579
NCBI Full Name:	Nuclear receptor coactivator 5
NCBI Synonym Full Names:	nuclear receptor coactivator 5
NCBI Official Symbol:	NCOA5
NCBI Official Synonym Symbols:	CIA; bA465L10.6
NCBI Protein Information:	nuclear receptor coactivator 5; NCoA-5; coactivator independent of AF-2
UniProt Protein Name:	Nuclear receptor coactivator 5
UniProt Synonym Protein Names:	Coactivator independent of AF-2; CIA
Protein Family:	Nuclear receptor coactivator
UniProt Gene Name:	NCOA5
UniProt Entry Name:	NCOA5_HUMAN

Western blot analysis of extracts of various cell lines, using NCOA5 antibody (CAB3476) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

NCOA5 Antibody (PACO04835)

NCOA5 Antibody (PACO10764)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)