Antibody Name:	Anti-NCOA2 Antibody [KO Validated]
Antibody SKU:	CAB10280
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 550-820 of human NCOA2 (NP_006531.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 550-820 of human NCOA2 (NP_006531.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SSLA SPDL KMGN LQNS PVNM NPPP LSKM GSLD SKDC FGLY GEPS EGTT GQAE SSCH PGEQ KETN DPNL PPAV SSER ADGQ SRLH DSKG QTKL LQLL TTKS DQME PSPL ASSL SDTN KDST GSLP GSGS THGT SLKE KHKI LHRL LQDS SSPV DLAK LTAE ATGK DLSQ ESSS TAPG SEVT IKQE PVSP KKKE NALL RYLL DKDD TKDI GLPE ITPK LERL DSKT DPAS NTKL IAMK TEKE EMSF EPGD QPGS ELDN LEEI LDDL QNSQ LPQ
Gene ID:	10499
Uniprot:	Q15596
Cellular Location:	Nucleus
Calculated MW:	159kDa
Observed MW:	200kDa

Synonyms:

NCOA2, GRIP1, KAT13C, NCoA-2, SRC2, TIF2, bHLHe75

Background:

The protein encoded by this gene functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SRC-2: a transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Present in a complex with CARM1 and p300. Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts with HIF1A, NCOA1, APEX and NR3C1. Interacts with CASP8AP2. Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the MYST3-NCOA2 oncogene, which consists of the N-terminus part of MYST3/MOZ and the C-terminus part of NCOA2/TIF2. MYST3-NCOA2 binds to CREBBP and disrupts its function in transcription activation. Phosphorylated and potentiated by the p38 MAPK signaling cascade in vitro and in vivo.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 8q13.3 Cellular Component: nuclear body; nucleoplasm; nucleus; protein complex Molecular Function:aryl hydrocarbon receptor binding; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; protein binding; protein domain specific binding; transcription coactivator activity Biological Process: bile acid and bile salt transport; cellular lipid metabolic process; cellular response to hormone stimulus; circadian regulation of gene expression; intracellular receptor-mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	The protein encoded by this gene functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
UniProt Code:	Q15596
NCBI GenInfo Identifier:	13626594
NCBI Gene ID:	10499
NCBI Accession:	Q15596.2
UniProt Secondary Accession:	Q15596,Q14CD2,
UniProt Related Accession:	Q15596
Molecular Weight:	159kDa
NCBI Full Name:	Nuclear receptor coactivator 2
NCBI Synonym Full Names:	nuclear receptor coactivator 2
NCBI Official Symbol:	NCOA2
NCBI Official Synonym Symbols:	SRC2; TIF2; GRIP1; KAT13C; NCoA-2; bHLHe75
NCBI Protein Information:	nuclear receptor coactivator 2
UniProt Protein Name:	Nuclear receptor coactivator 2
UniProt Synonym Protein Names:	Class E basic helix-loop-helix protein 75; bHLHe75; Transcriptional intermediary factor 2; hTIF2
Protein Family:	Nuclear receptor coactivator
UniProt Gene Name:	NCOA2

	Western blot analysis of extracts of various cell lines, using NCOA2 antibody (CAB10280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Western blot analysis of extracts from normal (control) and NCOA2 knockout (KO) 293T cells, using NCOA2 antibody (CAB10280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Immunohistochemistry of paraffin-embedded rat brain using NCOA2 antibody (CAB10280) at dilution of 1:100 (40x lens).