Antibody Name:	Anti-NCOA1 Antibody
Antibody SKU:	CAB1128
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1222-1441 of human NCOA1 (NP_003734.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	NIH/3T3, K-562

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1222-1441 of human NCOA1 (NP_003734.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NPQM QQNV FQYP GAGM VPQG EANF APSL SPGS SMVP MPIP PPQS SLLQ QTPP ASGY QSPD MKAW QQGA IGNN NVFS QAVQ NQPT PAQP GVYN NMSI TVSM AGGN TNVQ NMNP MMAQ MQMS SLQM PGMN TVCP EQIN DPAL RHTG LYCN QLSS TDLL KTEA DGTQ QVQQ VQVF ADVQ CTVN LVGG DPYL NQPG PLGT QKPT SGPQ TPQA QQKS LLQQ LLTE
Gene ID:	8648
Uniprot:	Q15788
Cellular Location:	Nucleus
Calculated MW:	152kDa/156kDa
Observed MW:	157kDa

Synonyms:

NCOA1, F-SRC-1, KAT13A, RIP160, SRC1, bHLHe42, bHLHe74

Background:

The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	SRC-1: a transcriptional coactivator for steroid and nuclear hormone receptors. A member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, and bHLH and PAS domains. Phosphorylated and activated via the mitogen-activated protein kinase pathway. Three alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:DNA-binding; Acetyltransferase; Oncoprotein; EC 2.3.1.48; Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 2p23 Cellular Component: nucleoplasm; neuron projection; nuclear chromatin; cytoplasm Molecular Function:ligand-dependent nuclear receptor binding; protein dimerization activity; transcription coactivator activity; protein N-terminus binding; retinoid X receptor binding; signal transducer activity; protein binding; histone acetyltransferase activity; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; androgen receptor binding; protein complex binding; estrogen receptor binding; chromatin binding; progesterone receptor binding; nuclear hormone receptor binding Biological Process: lactation; hypothalamus development; positive regulation of transcription from RNA polymerase II promoter by galactose; male mating behavior; intracellular receptor-mediated signaling pathway; response to retinoic acid; transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; male gonad development; hippocampus development; positive regulation of female receptivity; cellular lipid metabolic process; response to estradiol stimulus; cellular response to hormone stimulus; ovulation cycle; androgen receptor signaling pathway; cerebellum development; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; positive regulation of neuron differentiation; response to progesterone stimulus
NCBI Summary:	The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15788
NCBI GenInfo Identifier:	158518533
NCBI Gene ID:	8648
NCBI Accession:	Q15788.3
UniProt Related Accession:	Q15788
Molecular Weight:
NCBI Full Name:	Nuclear receptor coactivator 1
NCBI Synonym Full Names:	nuclear receptor coactivator 1
NCBI Official Symbol:	NCOA1
NCBI Official Synonym Symbols:	SRC1; KAT13A; RIP160; F-SRC-1; bHLHe42; bHLHe74
NCBI Protein Information:	nuclear receptor coactivator 1
UniProt Protein Name:	Nuclear receptor coactivator 1
UniProt Synonym Protein Names:	Class E basic helix-loop-helix protein 74; bHLHe74; Protein Hin-2; RIP160; Renal carcinoma antigen NY-REN-52; Steroid receptor coactivator 1; SRC-1
Protein Family:	Protein
UniProt Gene Name:	NCOA1
UniProt Entry Name:	NCOA1_HUMAN

	Western blot analysis of extracts of various cell lines, using NCOA1 antibody (CAB1128) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using NCOA1 antibody (CAB1128). Blue: DAPI for nuclear staining.