Immunology Antibodies 1
Anti-NCKAP1 Antibody (CAB12229)
- SKU:
- CAB12229
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-NCKAP1 Antibody |
| Antibody SKU: | CAB12229 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NCKAP1 (NP_038464.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, HeLa, MCF7, HepG2, Mouse heart, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NCKAP1 (NP_038464.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSRS VLQP SQQK LAEK LTIL NDRG VGML TRLY NIKK ACGD PKAK PSYL IDKN LESA VKFI VRKF PAVE TRNN NQQL AQLQ KEKS EILK NLAL YYFT FVDV MEFK DHVC ELLN TIDV CQVF FDIT VNFD LTKN YLDL IITY TTLM ILLS RIEE RKAI IGLY NYAH EMTH GASD REYP RLGQ MIVD YENP LKKM MEEF VPHS KSLS DALI SLQM VYPR RNLS ADQW RNAQ LLSL ISAP STML NPAQ SDTM PC |
| Gene ID: | 10787 |
| Uniprot: | Q9Y2A7 |
| Cellular Location: | Cell membrane, Cell projection, Cytoplasmic side, Single-pass membrane protein, lamellipodium membrane |
| Calculated MW: | 128kDa/129kDa |
| Observed MW: | 125kDa |
| Synonyms: | NCKAP1, HEM2, NAP1, NAP125, p125Nap1 |
| Background: |
| UniProt Protein Function: | NCKAP1: Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. Belongs to the HEM-1/HEM-2 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Apoptosis; Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 2q32 Cellular Component: focal adhesion; integral to membrane; cytosol Molecular Function:protein binding; Rac GTPase binding; protein complex binding Biological Process: lamellipodium biogenesis; somitogenesis; central nervous system development; basal protein localization; zygotic determination of anterior/posterior axis, embryo; protein stabilization; in utero embryonic development; apoptosis; Rac protein signal transduction; paraxial mesoderm morphogenesis; mesodermal cell migration; notochord morphogenesis; apical protein localization; embryonic body morphogenesis; regulation of protein localization; embryonic foregut morphogenesis; establishment and/or maintenance of actin cytoskeleton polarity; neural tube closure; embryonic heart tube development; innate immune response; vascular endothelial growth factor receptor signaling pathway; cell migration involved in gastrulation; endoderm development |
| UniProt Code: | Q9Y2A7 |
| NCBI GenInfo Identifier: | 12643947 |
| NCBI Gene ID: | 10787 |
| NCBI Accession: | Q9Y2A7.1 |
| UniProt Secondary Accession: | Q9Y2A7,O60329, Q53QN5, Q53S94, Q53Y35, |
| UniProt Related Accession: | Q9Y2A7 |
| Molecular Weight: | 129,517 Da |
| NCBI Full Name: | Nck-associated protein 1 |
| NCBI Synonym Full Names: | NCK-associated protein 1 |
| NCBI Official Symbol: | NCKAP1 |
| NCBI Official Synonym Symbols: | HEM2; NAP1; NAP125; p125Nap1 |
| NCBI Protein Information: | nck-associated protein 1; membrane-associated protein HEM-2 |
| UniProt Protein Name: | Nck-associated protein 1 |
| UniProt Synonym Protein Names: | Membrane-associated protein HEM-2; p125Nap1 |
| Protein Family: | Nck-associated protein |
| UniProt Gene Name: | NCKAP1 |
| UniProt Entry Name: | NCKP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NCKAP1 antibody (CAB12229) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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