Antibody Name:	Anti-NCK2 Antibody
Antibody SKU:	CAB7750
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human NCK2 (NP_003572.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human NCK2 (NP_003572.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTEE VIVI AKWD YTAQ QDQE LDIK KNER LWLL DDSK TWWR VRNA ANRT GYVP SNYV ERKN SLKK GSLV KNLK DTLG LGKT RRKT SARD ASPT PSTD AEYP ANGS GADR IYDL NIPA FVKF AYVA ERED ELSL VKGS RVTV MEKC SDGW WRGS YNGQ IGWF PSNY VLEE VDEA AAES PSFL SLRK GASL SNGQ GSRV LHVV QTLY PFSS VTEE ELNF EKGE TMEV IEKP ENDP EWWK CKNA RGQV GLVP KNYV VVLS DGPA LHPA HAPQ ISYT GPSS SGRF AGRE WYYG NVTR HQAE CALN ERGV EGDF LIRD SESS PSDF SVSL KASG KNKH FKVQ LVDN VYCI GQRR FHTM DELV EHYK KAPI FTSE HGEK LYLV RALQ
Gene ID:	8440
Uniprot:	O43639
Cellular Location:	Cytoplasm, Endoplasmic reticulum
Calculated MW:	42kDa
Observed MW:	43kDa

Synonyms:

NCK2, GRB4, NCKbeta

Background:

This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

UniProt Protein Function:	NCK2: Adapter protein which associates with tyrosine- phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1- dependent transcriptional activation in response to activated Ras signaling.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2q12 Cellular Component: endoplasmic reticulum; cytoplasm; vesicle membrane; cytosol Molecular Function:protein binding; receptor signaling complex scaffold activity; SH3/SH2 adaptor activity; cytoskeletal adaptor activity Biological Process: epidermal growth factor receptor signaling pathway; lamellipodium biogenesis; axon guidance; cell migration; T cell activation; positive regulation of signal transduction; regulation of epidermal growth factor receptor activity; negative regulation of peptidyl-serine phosphorylation; signal complex assembly; actin filament organization; signal transduction; regulation of translation; negative regulation of cell proliferation; positive regulation of actin filament polymerization; ephrin receptor signaling pathway; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway
NCBI Summary:	This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	O43639
NCBI GenInfo Identifier:	20532395
NCBI Gene ID:	8440
NCBI Accession:	O43639.2
UniProt Secondary Accession:	O43639,Q9BWN9, Q9UIC3, D3DVK1,
UniProt Related Accession:	O43639
Molecular Weight:	380
NCBI Full Name:	Cytoplasmic protein NCK2
NCBI Synonym Full Names:	NCK adaptor protein 2
NCBI Official Symbol:	NCK2
NCBI Official Synonym Symbols:	GRB4; NCKbeta
NCBI Protein Information:	cytoplasmic protein NCK2; SH2/SH3 adaptor protein NCK-beta; growth factor receptor-bound protein 4; noncatalytic region of tyrosine kinase, beta
UniProt Protein Name:	Cytoplasmic protein NCK2
UniProt Synonym Protein Names:	Growth factor receptor-bound protein 4; NCK adaptor protein 2; Nck-2; SH2/SH3 adaptor protein NCK-beta
Protein Family:	Cytoplasmic protein
UniProt Gene Name:	NCK2
UniProt Entry Name:	NCK2_HUMAN

	Western blot analysis of extracts of various cell lines, using NCK2 antibody (CAB7750) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human prostate using NCK2 antibody (CAB7750) at dilution of 1:100 (40x lens).