Cell Biology Antibodies 2
Anti-NCF1 Antibody (CAB1148)
- SKU:
- CAB1148
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NCF1 Antibody |
| Antibody SKU: | CAB1148 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-390 of human NCF1 (NP_000256.4). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | THP-1, Raji, HL-60, Mouse spleen, Mouse lung, Mouse liver, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-390 of human NCF1 (NP_000256.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGDT FIRH IALL GFEK RFVP SQHY VYMF LVKW QDLS EKVV YRRF TEIY EFHK TLKE MFPI EAGA INPE NRII PHLP APKW FDGQ RAAE NRQG TLTE YCST LMSL PTKI SRCP HLLD FFKV RPDD LKLP TDNQ TKKP ETYL MPKD GKST ATDI TGPI ILQT YRAI ANYE KTSG SEMA LSTG DVVE VVEK SESG WWFC QMKA KRGW IPAS FLEP LDSP DETE DPEP NYAG EPYV AIKA YTAV EGDE VSLL EGEA VEVI HKLL DGWW VIRK DDVT GYFP SMYL QKSG QDVS QAQR QIKR GAPP RRSS IRNA HSIH QRSR KRLS QDAY RRNS VRFL QQRR RQAR PGPQ SPGS PLEE ERQT QRSK PQPA VPPR PSAD LILN RCSE STKR KLAS AV |
| Gene ID: | 653361 |
| Uniprot: | P14598 |
| Cellular Location: | Cytoplasm, Cytoplasmic side, Membrane, Peripheral membrane protein, cytosol |
| Calculated MW: | 30kDa/44kDa |
| Observed MW: | 45kDa |
| Synonyms: | NCF1, NCF1A, NOXO2, SH3PXD1A, p47phox |
| Background: | The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. |
| UniProt Protein Function: | p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease. |
| UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.-.-.- Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: cytosol; extrinsic to membrane; NADPH oxidase complex Molecular Function:electron carrier activity; phosphatidylinositol-3,4-bisphosphate binding; phosphoinositide binding; protein binding; SH3 domain binding; superoxide-generating NADPH oxidase activity Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; cell proliferation; cell redox homeostasis; cellular defense response; hydrogen peroxide biosynthetic process; inflammatory response; innate immune response; leukotriene metabolic process; negative regulation of smooth muscle contraction; protein targeting to membrane; respiratory burst; respiratory burst during defense response; response to yeast; small GTPase mediated signal transduction; superoxide metabolic process; superoxide release; vascular endothelial growth factor receptor signaling pathway Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I |
| NCBI Summary: | The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P14598 |
| NCBI GenInfo Identifier: | 325511390 |
| NCBI Gene ID: | 653361 |
| NCBI Accession: | P14598.3 |
| UniProt Secondary Accession: | P14598,O43842, Q2PP07, Q53FR5, Q9BU90, Q9BXI7, Q9BXI8 Q9UDV9, Q9UMU2, A6NEH2, A8K7S9, |
| UniProt Related Accession: | P14598 |
| Molecular Weight: | |
| NCBI Full Name: | Neutrophil cytosol factor 1 |
| NCBI Synonym Full Names: | neutrophil cytosolic factor 1 |
| NCBI Official Symbol: | NCF1 |
| NCBI Official Synonym Symbols: | NCF1A; NOXO2; p47phox; SH3PXD1A |
| NCBI Protein Information: | neutrophil cytosol factor 1 |
| UniProt Protein Name: | Neutrophil cytosol factor 1 |
| UniProt Synonym Protein Names: | 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox |
| Protein Family: | Neutrophil cytosol factor |
| UniProt Gene Name: | NCF1 |
| UniProt Entry Name: | NCF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NCF1 antibody (CAB1148) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunoprecipitation analysis of 200ug extracts of Raji cells, using 3 ug NCF1 antibody (CAB1148). Western blot was performed from the immunoprecipitate using NCF1 antibody (CAB1148) at a dilition of 1:1000. |
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