Antibody Name:	Anti-NCBP2 Antibody
Antibody SKU:	CAB7293
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human NCBP2 (NP_031388.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	MCF7, Jurkat, SKOV3, HeLa, NIH/3T3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human NCBP2 (NP_031388.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSGG LLKA LRSD SYVE LSQY RDQH FRGD NEEQ EKLL KKSC TLYV GNLS FYTT EEQI YELF SKSG DIKK IIMG LDKM KKTA CGFC FVEY YSRA DAEN AMRY INGT RLDD RIIR TDWD AGFK EGRQ YGRG RSGG QVRD EYRQ DYDA GRGG YGKL AQNQ
Gene ID:	22916
Uniprot:	P52298
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	11kDa/15kDa/18kDa
Observed MW:	14kDa

Synonyms:

NCBP2, CBC2, CBP20, NIP1, PIG55

Background:

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	NCBP2: Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA- mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. Belongs to the RRM NCBP2 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Translation; RNA-binding; Spliceosome; RNA splicing Chromosomal Location of Human Ortholog: 3q29 Cellular Component: cytoplasm; cytosol; mRNA cap complex; nucleoplasm; nucleus Molecular Function:mRNA binding; nucleotide binding; protein binding; RNA 7-methylguanosine cap binding; RNA binding; RNA cap binding; snRNA binding Biological Process: gene expression; histone mRNA metabolic process; mRNA 3'-end processing; mRNA capping; mRNA catabolic process, nonsense-mediated decay; mRNA export from nucleus; nuclear mRNA cis splicing, via U2-type spliceosome; nuclear mRNA splicing, via spliceosome; positive regulation of mRNA 3'-end processing; positive regulation of RNA export from nucleus; positive regulation of viral transcription; regulation of translational initiation; RNA elongation from RNA polymerase II promoter; RNA splicing; RNA-mediated gene silencing; snRNA export from nucleus; spliceosomal snRNP biogenesis; termination of RNA polymerase II transcription; transcription from RNA polymerase II promoter; viral reproduction
NCBI Summary:	The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P52298
NCBI GenInfo Identifier:	1705651
NCBI Gene ID:	22916
NCBI Accession:	P52298.1
UniProt Secondary Accession:	P52298,Q14924, Q2TS50, B2RE91, B4DMK7, E9PAR5,
UniProt Related Accession:	P52298
Molecular Weight:	11,932 Da
NCBI Full Name:	Nuclear cap-binding protein subunit 2
NCBI Synonym Full Names:	nuclear cap binding protein subunit 2
NCBI Official Symbol:	NCBP2
NCBI Official Synonym Symbols:	CBC2; NIP1; CBP20; PIG55
NCBI Protein Information:	nuclear cap-binding protein subunit 2
UniProt Protein Name:	Nuclear cap-binding protein subunit 2
UniProt Synonym Protein Names:	20 kDa nuclear cap-binding protein; Cell proliferation-inducing gene 55 protein; NCBP 20 kDa subunit; CBP20; NCBP-interacting protein 1; NIP1
Protein Family:	Nuclear cap-binding protein
UniProt Gene Name:	NCBP2
UniProt Entry Name:	NCBP2_HUMAN

	Western blot analysis of extracts of various cell lines, using NCBP2 antibody (CAB7293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon using NCBP2 antibody (CAB7293) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using NCBP2 antibody (CAB7293) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using NCBP2 antibody (CAB7293). Blue: DAPI for nuclear staining.