Antibody Name:	Anti-NAT8 Antibody
Antibody SKU:	CAB7759
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human NAT8

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Mouse spleen, Mouse testis, Mouse lung, Rat kidney

Immunogen:	Recombinant protein of human NAT8
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	9027
Uniprot:	Q9UHE5
Cellular Location:	Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Single-pass type II membrane protein
Calculated MW:	25kDa
Observed MW:	26kDa

Synonyms:

NAT8, ATase2, CCNAT, CML1, GLA, Hcml1, TSC501, TSC510

Background:

This gene, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene.

UniProt Protein Function:	NAT8: Plays a role in regulation of gastrulation. Belongs to the camello family.
UniProt Protein Details:	Protein type:EC 2.3.1.80; Membrane protein, integral; Cell adhesion; Membrane protein, multi-pass; Acetyltransferase Chromosomal Location of Human Ortholog: 2p13.2 Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment; ER-Golgi intermediate compartment membrane; integral to membrane Molecular Function:cysteine-S-conjugate N-acetyltransferase activity; lysine N-acetyltransferase activity; protein binding Biological Process: beta-amyloid metabolic process; cellular protein metabolic process; glutathione metabolic process; negative regulation of apoptosis; peptidyl-lysine N6-acetylation; response to drug
NCBI Summary:	This gene, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene. [provided by RefSeq, Sep 2008]
UniProt Code:	Q9UHE5
NCBI GenInfo Identifier:	145566894
NCBI Gene ID:	9027
NCBI Accession:	Q9UHE5.2
UniProt Secondary Accession:	Q9UHE5,O75839, Q6LEU4, Q96QI8, Q9UQ17,
UniProt Related Accession:	Q9UHE5
Molecular Weight:	25,619 Da
NCBI Full Name:	N-acetyltransferase 8
NCBI Synonym Full Names:	N-acetyltransferase 8 (putative)
NCBI Official Symbol:	NAT8
NCBI Official Synonym Symbols:	GLA; CML1; CCNAT; Hcml1; ATase2; TSC501; TSC510
NCBI Protein Information:	N-acetyltransferase 8
UniProt Protein Name:	N-acetyltransferase 8
UniProt Synonym Protein Names:	Acetyltransferase 2; ATase2; Camello-like protein 1; Cysteinyl-conjugate N-acetyltransferase (EC:2.3.1.80); CCNAT
UniProt Gene Name:	NAT8
UniProt Entry Name:	NAT8_HUMAN

	Western blot analysis of extracts of various cell lines, using NAT8 antibody (CAB7759) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using NAT8 antibody (CAB7759) (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using NAT8 antibody (CAB7759) (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using NAT8 antibody (CAB7759) (40x lens).