Epigenetics & Nuclear Signaling Antibodies 4
Anti-NAT10 Antibody (CAB7292)
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- SKU:
- CAB7292
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-NAT10 Antibody |
| Antibody SKU: | CAB7292 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NAT10 (NP_078938.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Rat |
| Positive Samples: | HepG2, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NAT10 (NP_078938.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MHRK KVDN RIRI LIEN GVAE RQRS LFVV VGDR GKDQ VVIL HHML SKAT VKAR PSVL WCYK KELG FSSH RKKR MRQL QKKI KNGT LNIK QDDP FELF IAAT NIRY CYYN ETHK ILGN TFGM CVLQ DFEA LTPN LLAR TVET VEGG GLVV ILLR TMNS LKQL YTVT MDVH SRYR TEAH QDVV GRFN ERFI LSLA SCKK CLVI DDQL NILP ISSH VATM EALP PQTP DESL GPSD LELR ELKE SLQD TQPV GV |
| Gene ID: | 55226 |
| Uniprot: | Q9H0A0 |
| Cellular Location: | Nucleus, nucleolus |
| Calculated MW: | 107kDa/115kDa |
| Observed MW: | 116kDa |
| Synonyms: | NAT10, ALP, NET43, Kre33 |
| Background: | The protein encoded by this gene is an RNA cytidine acetyltransferase involved in histone acetylation, tRNA acetylation, the biosynthesis of 18S rRNA, and the enhancement of nuclear architecture and chromatin organization. |
| UniProt Protein Function: | ALP: Has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division. Belongs to the UPF0202 family. |
| UniProt Protein Details: | Protein type:EC 2.3.1.-; Acetyltransferase; Nucleolus Chromosomal Location of Human Ortholog: 11p13 Cellular Component: membrane; nucleolus; nucleus Molecular Function:N-acetyltransferase activity; ATP binding Biological Process: metabolic process |
| NCBI Summary: | The protein encoded by this gene is an RNA cytidine acetyltransferase involved in histone acetylation, tRNA acetylation, the biosynthesis of 18S rRNA, and the enhancement of nuclear architecture and chromatin organization. [provided by RefSeq, Oct 2016] |
| UniProt Code: | Q9H0A0 |
| NCBI GenInfo Identifier: | 313104140 |
| NCBI Gene ID: | 55226 |
| NCBI Accession: | Q9H0A0.2 |
| UniProt Related Accession: | Q9H0A0 |
| Molecular Weight: | |
| NCBI Full Name: | RNA cytidine acetyltransferase |
| NCBI Synonym Full Names: | N-acetyltransferase 10 |
| NCBI Official Symbol: | NAT10 |
| NCBI Official Synonym Symbols: | ALP; Kre33; NET43 |
| NCBI Protein Information: | RNA cytidine acetyltransferase |
| UniProt Protein Name: | N-acetyltransferase 10 |
| Protein Family: | Putative nucleobase-ascorbate transporter |
| UniProt Gene Name: | NAT10 |
| UniProt Entry Name: | NAT10_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NAT10 antibody (CAB7292) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunofluorescence analysis of MCF-7 cells using NAT10 antibody (CAB7292). Blue: DAPI for nuclear staining. |
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