Cell Cycle Antibodies 2

Anti-NASP Antibody (CAB6938)

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SKU:
CAB6938
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-NASP Antibody
Antibody SKU:CAB6938
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-449 of human NASP (NP_689511.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SW620, HL-60, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-449 of human NASP (NP_689511.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:WDML DLAK IIFK RQET KEAQ LYAA QAHL KLGE VSVE SENY VQAV EEFQ SCLN LQEQ YLEA HDRL LAET HYQL GLAY GYNS QYDE AVAQ FSKS IEVI ENRM AVLN EQVK EAEG SSAE YKKE IEEL KELL PEIR EKIE DAKE SQRS GNVA ELAL KATL VESS TSGF TPGG GGSS VSMI ASRK PTDG ASSS NCVT DISH LVRK KRKP EEES PRKD DAKK AKQE PEVN GGSG DAVP SGNE VSEN MEEE AENQ AESR AAVE GTVE AGAT VEST AC
Gene ID:4678
Uniprot:P49321
Cellular Location:Cytoplasm, Nucleus
Calculated MW:48kDa/78kDa/85kDa/86kDa
Observed MW:85kDa
Synonyms:NASP, FLB7527, HMDRA1, PRO1999
Background:This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
UniProt Protein Function:NASP: Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA. Belongs to the NASP family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: nucleoplasm; protein complex; cytoplasm; nuclear chromatin; nucleus

Molecular Function:histone binding; Hsp90 protein binding

Biological Process: cell proliferation; blastocyst development; protein transport; DNA replication-independent nucleosome assembly; histone exchange; cell cycle; DNA replication; DNA replication-dependent nucleosome assembly

UniProt Code:P49321
NCBI GenInfo Identifier:23503077
NCBI Gene ID:
NCBI Accession:P49321.2
Molecular Weight:~ 85kDa
NCBI Full Name:Nuclear autoantigenic sperm protein
UniProt Protein Name:Nuclear autoantigenic sperm protein
Protein Family:NASP-related protein
UniProt Gene Name:NASP  
UniProt Entry Name:NASP_HUMAN
Anti-NASP Antibody (CAB6938)Western blot analysis of extracts of various cell lines, using NASP antibody (CAB6938) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-NASP Antibody (CAB6938)Immunohistochemistry of paraffin-embedded rat spleen using NASP Antibody (CAB6938) at dilution of 1:100 (40x lens).
Anti-NASP Antibody (CAB6938)Immunohistochemistry of paraffin-embedded human brain astrocytoma using NASP Antibody (CAB6938) at dilution of 1:100 (40x lens).
Anti-NASP Antibody (CAB6938)Immunohistochemistry of paraffin-embedded mouse spleen using NASP Antibody (CAB6938) at dilution of 1:100 (40x lens).
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