Metabolism Antibodies 1

Anti-NAPRT Antibody (CAB11591)

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SKU:
CAB11591
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-NAPRT Antibody
Antibody SKU:CAB11591
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 309-538 of human NAPRT1 (NP_660202.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, HT-29
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 309-538 of human NAPRT1 (NP_660202.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ELGY RAVG VRLD SGDL LQQA QEIR KVFR AAAA QFQV PWLE SVLI VVSN NIDE EALA RLAQ EGSE VNVI GIGT SVVT CPQQ PSLG GVYK LVAV GGQP RMKL TEDP EKQT LPGS KAAF RLLG SDGS PLMD MLQL AEEP VPQA GQEL RVWP PGAQ EPCT VRPA QVEP LLRL CLQQ GQLC EPLP SLAE SRAL AQLS LSRL SPEH RRLR SPAQ YQVV LSER LQAL VNSL CAGQ SP
Gene ID:93100
Uniprot:Q6XQN6
Cellular Location:Cytoplasm, cytosol
Calculated MW:56kDa/57kDa/60kDa
Observed MW:55kDa
Synonyms:NAPRT, NAPRT1, PP3856
Background:
UniProt Protein Function:NAPRT1: Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. Belongs to the NAPRTase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 6.3.4.21; Transferase

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol; extracellular region

Molecular Function:nicotinate phosphoribosyltransferase activity; protein binding

Biological Process: neutrophil degranulation; response to oxidative stress

NCBI Summary:Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM, Mar 2008]
UniProt Code:Q6XQN6
NCBI GenInfo Identifier:166221824
NCBI Gene ID:93100
NCBI Accession:Q6XQN6.2
UniProt Secondary Accession:Q6XQN6,Q6PJL1, Q6XQN4, Q6XQN5, Q8N5E8, Q9BRG0, A7BFI3
UniProt Related Accession:Q6XQN6
Molecular Weight:58kDa
NCBI Full Name:Nicotinate phosphoribosyltransferase
NCBI Synonym Full Names:nicotinate phosphoribosyltransferase
NCBI Official Symbol:NAPRT  
NCBI Official Synonym Symbols:NAPRT1; PP3856  
NCBI Protein Information:nicotinate phosphoribosyltransferase
UniProt Protein Name:Nicotinate phosphoribosyltransferase
UniProt Synonym Protein Names:FHA-HIT-interacting protein; Nicotinate phosphoribosyltransferase domain-containing protein 1
UniProt Gene Name:NAPRT  
Anti-NAPRT Antibody (CAB11591)Western blot analysis of extracts of various cell lines, using NAPRT Antibody (CAB11591) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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