Cell Biology Antibodies 10
Anti-NAPG Antibody (CAB6432)
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- SKU:
- CAB6432
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NAPG Antibody |
| Antibody SKU: | CAB6432 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human NAPG (NP_003817.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, SW480, HepG2, HeLa, U-251MG, Mouse brain, Mouse spleen, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human NAPG (NP_003817.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAQ KINE GLEH LAKA EKYL KTGF LKWK PDYD SAAS EYGK AAVA FKNA KQFE QAKD ACLR EAVA HENN RALF HAAK AYEQ AGMM LKEM QKLP EAVQ LIEK ASMM YLEN GTPD TAAM ALER AGKL IENV DPEK AVQL YQQT ANVF ENEE RLRQ AVEL LGKA SRLL VRGR RFDE AALS IQKE KNIY KEIE NYPT CYKK TIAQ VLVH LHRN DYVA AERC VRES YSIP GFNG SEDC AALE QLLE GYDQ QDQD QVSD VCNS PLFK YMDN DYAK LGLS LVVP GGGI KKKS PATP QAKP DGVT ATAA DEEE DEYS GGLC |
| Gene ID: | 8774 |
| Uniprot: | Q99747 |
| Cellular Location: | Membrane, Peripheral membrane protein |
| Calculated MW: | 25kDa/34kDa |
| Observed MW: | 36kDa |
| Synonyms: | NAPG, GAMMASNAP |
| Background: | This gene encodes soluble NSF attachment protein gamma. The soluble NSF attachment proteins (SNAPs) enable N-ethyl-maleimide-sensitive fusion protein (NSF) to bind to target membranes. NSF and SNAPs appear to be general components of the intracellular membrane fusion apparatus, and their action at specific sites of fusion must be controlled by SNAP receptors particular to the membranes being fused. The product of this gene mediates platelet exocytosis and controls the membrane fusion events of this process. |
| UniProt Protein Function: | SNAP-gamma: a cytoplasmic peripheral membrane protein that mediates platelet exocytosis and controls the membrane fusion events of this process. Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 18p11.22 Cellular Component: lysosomal membrane; mitochondrion; SNARE complex Molecular Function:protein binding; soluble NSF attachment protein activity; syntaxin binding Biological Process: intra-Golgi vesicle-mediated transport; intracellular protein transport |
| NCBI Summary: | This gene encodes soluble NSF attachment protein gamma. The soluble NSF attachment proteins (SNAPs) enable N-ethyl-maleimide-sensitive fusion protein (NSF) to bind to target membranes. NSF and SNAPs appear to be general components of the intracellular membrane fusion apparatus, and their action at specific sites of fusion must be controlled by SNAP receptors particular to the membranes being fused. The product of this gene mediates platelet exocytosis and controls the membrane fusion events of this process.[provided by RefSeq, Dec 2008] |
| UniProt Code: | Q99747 |
| NCBI GenInfo Identifier: | 3024632 |
| NCBI Gene ID: | 8774 |
| NCBI Accession: | Q99747.1 |
| UniProt Secondary Accession: | Q99747,Q9BUV1, B4DFC9, |
| UniProt Related Accession: | Q99747 |
| Molecular Weight: | 25,662 Da |
| NCBI Full Name: | Gamma-soluble NSF attachment protein |
| NCBI Synonym Full Names: | NSF attachment protein gamma |
| NCBI Official Symbol: | NAPG |
| NCBI Official Synonym Symbols: | GAMMASNAP |
| NCBI Protein Information: | gamma-soluble NSF attachment protein |
| UniProt Protein Name: | Gamma-soluble NSF attachment protein |
| UniProt Synonym Protein Names: | N-ethylmaleimide-sensitive factor attachment protein gamma |
| Protein Family: | Ferredoxin-type protein |
| UniProt Gene Name: | NAPG |
| UniProt Entry Name: | SNAG_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NAPG antibody (CAB6432) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using NAPG antibody (CAB6432). Blue: DAPI for nuclear staining. |
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