Epigenetics & Nuclear Signaling Antibodies 3

Anti-NANOG Antibody (CAB3232)

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SKU:
CAB3232
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

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Antibody Name:Anti-NANOG Antibody
Antibody SKU:CAB3232
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human NANOG (NP_079141.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Mouse
Positive Samples:NIH/3T3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human NANOG (NP_079141.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSVD PACP QSLP CFEA SDCK ESSP MPVI CGPE ENYP SLQM SSAE MPHT ETVS PLPS SMDL LIQD SPDS STSP KGKQ PTSA EKSV AKKE DKVP VKKQ KTRT
Gene ID:79923
Uniprot:Q9H9S0
Cellular Location:Nucleus
Calculated MW:32kDa/34kDa
Observed MW:48kDa
Synonyms:NANOG
Background:The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:NANOG: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. Interacts with SMAD1 and SALL4. Expressed in testicular carcinoma and derived germ cell tumors. Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Belongs to the Nanog homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function:DNA binding; chromatin binding; transcription factor activity; transcription corepressor activity

Biological Process: response to retinoic acid; gonad development; transcription, DNA-dependent; somatic stem cell maintenance; stem cell division; endodermal cell fate specification; stem cell maintenance; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; embryonic pattern specification; mesodermal cell fate commitment; negative regulation of cell fate commitment; negative regulation of BMP signaling pathway; cell proliferation; regulation of transcription, DNA-dependent; regulation of gene expression; regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation

NCBI Summary:The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
UniProt Code:Q9H9S0
NCBI GenInfo Identifier:118573073
NCBI Gene ID:79923
NCBI Accession:Q9H9S0.2
UniProt Secondary Accession:Q9H9S0,Q2TTG0, Q6JZS5, D3DUU4,
UniProt Related Accession:Q9H9S0
Molecular Weight:305
NCBI Full Name:Homeobox protein NANOG
NCBI Synonym Full Names:Nanog homeobox
NCBI Official Symbol:NANOG  
NCBI Protein Information:homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
UniProt Protein Name:Homeobox protein NANOG
UniProt Synonym Protein Names:Homeobox transcription factor Nanog; hNanog
Protein Family:NANOG neighbor homeobox
UniProt Gene Name:NANOG  
UniProt Entry Name:NANOG_HUMAN
Anti-NANOG Antibody (CAB3232)Western blot analysis of extracts of NIH/3T3 cells, using NANOG antibody (CAB3232) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
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