Cell Biology Antibodies 12

Anti-NAGK Antibody (CAB9070)

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SKU:
CAB9070
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NAGK Antibody
Antibody SKU:CAB9070
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 50-210 of human NAGK (NP_060037.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, A-431, Jurkat, SW480, Mouse brain, Mouse kidney, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 50-210 of human NAGK (NP_060037.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IYGG VEGG GTRS EVLL VSED GKIL AEAD GLST NHWL IGTD KCVE RINE MVNR AKRK AGVD PLVP LRSL GLSL SGGD QEDA GRIL IEEL RDRF PYLS ESYL ITTD AAGS IATA TPDG GVVL ISGT GSNC RLIN PDGS ESGC GGWG HMMG DEGS AYWI AHQA V
Gene ID:55577
Uniprot:Q9UJ70
Cellular Location:
Calculated MW:37kDa/42kDa
Observed MW:37kDa
Synonyms:NAGK, GNK, HSA242910
Background:This gene encodes a member of the N-acetylhexosamine kinase family. The encoded protein catalyzes the conversion of N-acetyl-D-glucosamine to N-acetyl-D-glucosamine 6-phosphate, and is the major mammalian enzyme which recovers amino sugars.
UniProt Protein Function:NAGK: Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity. Belongs to the eukaryotic-type N-acetylglucosamine kinase family.
UniProt Protein Details:

Protein type:EC 2.7.1.59; Kinase, other; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 2p13.3

Molecular Function:protein binding; N-acetylglucosamine kinase activity; ATP binding; N-acylmannosamine kinase activity

Biological Process: N-acetylneuraminate catabolic process; N-acetylglucosamine metabolic process; carbohydrate phosphorylation; N-acetylmannosamine metabolic process

NCBI Summary:This gene encodes a member of the N-acetylhexosamine kinase family. The encoded protein catalyzes the conversion of N-acetyl-D-glucosamine to N-acetyl-D-glucosamine 6-phosphate, and is the major mammalian enzyme which recovers amino sugars. [provided by RefSeq, Nov 2011]
UniProt Code:Q9UJ70
NCBI GenInfo Identifier:48146659
NCBI Gene ID:55577
NCBI Accession:CAG33552.1
UniProt Secondary Accession:Q9UJ70,Q53HD5, Q6IA84, Q9BS29, Q9BVP0, Q9NV37, B4DLZ5
UniProt Related Accession:Q9UJ70
Molecular Weight:37,376 Da
NCBI Full Name:NAGK
NCBI Synonym Full Names:N-acetylglucosamine kinase
NCBI Official Symbol:NAGK  
NCBI Official Synonym Symbols:GNK; HSA242910  
NCBI Protein Information:N-acetyl-D-glucosamine kinase; glcNAc kinase
UniProt Protein Name:N-acetyl-D-glucosamine kinase
UniProt Synonym Protein Names:GlcNAc kinase
Protein Family:N-acetyl-D-glucosamine kinase
UniProt Gene Name:NAGK  
UniProt Entry Name:NAGK_HUMAN
Anti-NAGK Polyclonal Antibody (CAB9070)Western blot analysis of extracts of various cell lines, using NAGK antibody (CAB9070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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