Cell Biology Antibodies 12
Anti-NADPH oxidase 1 Antibody (CAB8527)
- SKU:
- CAB8527
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NADPH oxidase 1 Antibody |
| Antibody SKU: | CAB8527 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 415-564 of human NOX1 (NP_008983.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, SW480, Mouse liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 415-564 of human NOX1 (NP_008983.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KSIW YKFQ CADH NLKT KKIY FYWI CRET GAFS WFNN LLTS LEQE MEEL GKVG FLNY RLFL TGWD SNIV GHAA LNFD KATD IVTG LKQK TSFG RPMW DNEF STIA TSHP KSVV GVFL CGPR TLAK SLRK CCHR YSSL DPRK VQFY FNKE NF |
| Gene ID: | 27035 |
| Uniprot: | Q9Y5S8 |
| Cellular Location: | Cell projection, Multi-pass membrane protein, invadopodium membrane |
| Calculated MW: | 21kDa/58kDa/64kDa |
| Observed MW: | 65kDa |
| Synonyms: | NOX1, GP91-2, MOX1, NOH-1, NOH1 |
| Background: | This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | NOX1: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Channel, misc.; EC 1.-.-.-; Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Oxidoreductase Chromosomal Location of Human Ortholog: Xq22 Cellular Component: early endosome; integral to membrane; cell junction; NADPH oxidase complex Molecular Function:protein binding; metal ion binding; superoxide-generating NADPH oxidase activity; Rac GTPase binding; NADP binding; voltage-gated proton channel activity Biological Process: respiratory burst; intracellular pH elevation; cell migration; extracellular matrix organization and biogenesis; positive regulation of smooth muscle cell proliferation; superoxide metabolic process; positive regulation of JNK cascade; signal transduction; regulation of systemic arterial blood pressure by renin-angiotensin; NADP metabolic process; proton transport; regulation of blood pressure; positive regulation of cell proliferation; hydrogen peroxide metabolic process; angiogenesis; positive regulation of integrin biosynthetic process; inflammatory response; superoxide release |
| NCBI Summary: | This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012] |
| UniProt Code: | Q9Y5S8 |
| NCBI GenInfo Identifier: | 8134597 |
| NCBI Gene ID: | 27035 |
| NCBI Accession: | Q9Y5S8.2 |
| UniProt Secondary Accession: | Q9Y5S8,O95691, Q2PP02, A8K836, |
| UniProt Related Accession: | Q9Y5S8 |
| Molecular Weight: | 58,972 Da |
| NCBI Full Name: | NADPH oxidase 1 |
| NCBI Synonym Full Names: | NADPH oxidase 1 |
| NCBI Official Symbol: | NOX1 |
| NCBI Official Synonym Symbols: | MOX1; NOH1; NOH-1; GP91-2 |
| NCBI Protein Information: | NADPH oxidase 1; mitogenic oxidase 1; NADPH oxidase homolog-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating) |
| UniProt Protein Name: | NADPH oxidase 1 |
| UniProt Synonym Protein Names: | Mitogenic oxidase 1; MOX-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; NOH-1 |
| Protein Family: | NADPH oxidase |
| UniProt Gene Name: | NOX1 |
| UniProt Entry Name: | NOX1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NOX1 antibody (CAB8527) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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