Name: Anti-NACC1 Antibody (CAB11720)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB11720
Availability: OutOfStock

Antibody Name:	Anti-NACC1 Antibody
Antibody SKU:	CAB11720
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-340 of human NACC1 (NP_443108.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SKOV3, Hela, Jurkat, Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-340 of human NACC1 (NP_443108.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QQES DSVQ CMPV AKRL WDSG QKEA GGGG NGSR KMAK FSTP DLAA NRPH QPPP PQQA PVVA AAQP AVAA GAGQ PAGG VAAA GGVV SGPS TSER TSPG TSSA YTSD SPGS YHNE EDEE EDGG EEGM DEQY RQIC NMYT MYSM MNVG QTAE KVEA LPEQ VAPE SRNR IRVR QDL
Gene ID:	112939
Uniprot:	Q96RE7
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	57kDa
Observed MW:	57kDa

Synonyms:

NACC1, BEND8, BTBD14B, BTBD30, NAC-1, NAC1, NECFM

Background:

This gene encodes a member of the BTB/POZ protein family. BTB/POZ proteins are involved in several cellular processes including proliferation, apoptosis and transcription regulation. The encoded protein is a transcriptional repressor that plays a role in stem cell self-renewal and pluripotency maintenance. The encoded protein also suppresses transcription of the candidate tumor suppressor Gadd45GIP1, and expression of this gene may play a role in the progression of multiple types of cancer. A pseudogene of this gene is located on the short arm of chromosome 9.

UniProt Protein Function:	NAC1: Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines. Homooligomer; mediated by the BTB domain. Interacts with HDAC3 and HDAC4. Interacts (via BTB domain) with CUL3, PSMD7 AND RCOR1. Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.
UniProt Protein Details:	Protein type:Transcription factor Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: nuclear body; nucleus Biological Process: negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; protein homooligomerization
NCBI Summary:	This gene encodes a member of the BTB/POZ protein family. BTB/POZ proteins are involved in several cellular processes including proliferation, apoptosis and transcription regulation. The encoded protein is a transcriptional repressor that plays a role in stem cell self-renewal and pluripotency maintenance. The encoded protein also suppresses transcription of the candidate tumor suppressor Gadd45GIP1, and expression of this gene may play a role in the progression of multiple types of cancer. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Feb 2012]
UniProt Code:	Q96RE7
NCBI GenInfo Identifier:	74732694
NCBI Gene ID:	112939
NCBI Accession:	Q96RE7.1
UniProt Related Accession:	Q96RE7
Molecular Weight:	57,258 Da
NCBI Full Name:	Nucleus accumbens-associated protein 1
NCBI Synonym Full Names:	nucleus accumbens associated 1
NCBI Official Symbol:	NACC1
NCBI Official Synonym Symbols:	NAC1; BEND8; NAC-1; BTBD30; BTBD14B
NCBI Protein Information:	nucleus accumbens-associated protein 1
UniProt Protein Name:	Nucleus accumbens-associated protein 1
UniProt Synonym Protein Names:	BTB/POZ domain-containing protein 14B
Protein Family:	Nucleus accumbens-associated protein
UniProt Gene Name:	NACC1
UniProt Entry Name:	NACC1_HUMAN

Western blot analysis of extracts of various cell lines, using NACC1 Antibody (CAB11720) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

Anti-NACC1 Antibody (CAB11709)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)