Cell Biology Antibodies 10
Anti-NAA20 Antibody (CAB7105)
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- SKU:
- CAB7105
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NAA20 Antibody |
| Antibody SKU: | CAB7105 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 49-178 of human NAA20 (NP_057184.1). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Mouse brain, Mouse kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 49-178 of human NAA20 (NP_057184.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PGGE LMGY IMGK AEGS VARE EWHG HVTA LSVA PEFR RLGL AAKL MELL EEIS ERKG GFFV DLFV RVSN QVAV NMYK QLGY SVYR TVIE YYSA SNGE PDED AYDM RKAL SRDT EKKS IIPL PHPV RPED IE |
| Gene ID: | 51126 |
| Uniprot: | P61599 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 12kDa/20kDa |
| Observed MW: | 20kDa |
| Synonyms: | NAA20, NAT3, NAT3P, NAT5, NAT5P, dJ1002M8.1 |
| Background: | NAT5 is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminal acetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008 [PubMed 18570629]).[supplied by OMIM, Apr 2009] |
| UniProt Protein Function: | NAA20: Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration. Belongs to the acetyltransferase family. ARD1 subfamily. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - phenylalanine; Acetyltransferase; Lipid Metabolism - glycerophospholipid; EC 2.3.1.88; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - tyrosine Chromosomal Location of Human Ortholog: 20p11.23 Cellular Component: cytoplasm; intracellular; nucleus Molecular Function:peptide alpha-N-acetyltransferase activity Biological Process: N-terminal peptidyl-methionine acetylation |
| NCBI Summary: | NAT5 is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminal acetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008 [PubMed 18570629]).[supplied by OMIM, Apr 2009] |
| UniProt Code: | P61599 |
| NCBI GenInfo Identifier: | 47606438 |
| NCBI Gene ID: | 51126 |
| NCBI Accession: | P61599.1 |
| UniProt Related Accession: | P61599 |
| Molecular Weight: | Predicted Molecular Mass: 24.1kDaAccurate Molecular Mass: 24kDa |
| NCBI Full Name: | N-alpha-acetyltransferase 20 |
| NCBI Synonym Full Names: | N-alpha-acetyltransferase 20, NatB catalytic subunit |
| NCBI Official Symbol: | NAA20 |
| NCBI Official Synonym Symbols: | NAT3; NAT5; NAT3P; NAT5P; dJ1002M8.1 |
| NCBI Protein Information: | N-alpha-acetyltransferase 20 |
| UniProt Protein Name: | N-alpha-acetyltransferase 20 |
| UniProt Synonym Protein Names: | Methionine N-acetyltransferase; N-acetyltransferase 5; N-terminal acetyltransferase B complex catalytic subunit NAA20; N-terminal acetyltransferase B complex catalytic subunit NAT5; NatB complex subunit NAT5; NatB catalytic subunit |
| Protein Family: | Nucleobase-ascorbate transporter |
| UniProt Gene Name: | NAA20 |
| UniProt Entry Name: | NAA20_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NAA20 antibody (CAB7105) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using NAA20 antibody (CAB7105) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human oophoroma using NAA20 antibody (CAB7105) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A-549 cells using NAA20 antibody (CAB7105). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug NAA20 antibody (CAB7105). Western blot was performed from the immunoprecipitate using NAA20 antibody (CAB7105) at a dilition of 1:1000. |
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