Signal Transduction Antibodies 3
Anti-Na+/K+-ATPase Antibody (CAB7878)
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- SKU:
- CAB7878
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-Na+/K+-ATPase Antibody |
| Antibody SKU: | CAB7878 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Na+/K+-ATPase (NP_000692.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, 293T, C2C12, C6 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Na+/K+-ATPase (NP_000692.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGKG VGRD KYEP AAVS EQGD KKGK KGKK DRDM DELK KEVS MDDH KLSL DELH RKYG TDLS RGLT SARA AEIL ARDG PNAL TPPP TTPE WIKF CRQL FGGF |
| Gene ID: | 476 |
| Uniprot: | P05023 |
| Cellular Location: | Cell membrane, Melanosome, Multi-pass membrane protein |
| Calculated MW: | 74kDa/109kDa/112kDa/113kDa |
| Observed MW: | 100KDa |
| Synonyms: | ATP1A1, ATPase Na+/K+ transporting subunit alpha 1, ATP1A, CMT2DD, HOMGSMR2 |
| Background: | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ATP1A1: the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients. Two splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:EC 3.6.3.9; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel Chromosomal Location of Human Ortholog: 1p13.1 Cellular Component: endoplasmic reticulum; Golgi apparatus; integral to membrane; membrane; plasma membrane; protein complex; sarcolemma; sodium:potassium-exchanging ATPase complex Molecular Function:ATP binding; chaperone binding; potassium ion binding; protein binding; sodium ion binding; sodium:potassium-exchanging ATPase activity Biological Process: cellular potassium ion homeostasis; cellular sodium ion homeostasis; potassium ion import; regulation of sodium ion transport |
| NCBI Summary: | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| UniProt Code: | P05023 |
| NCBI GenInfo Identifier: | 114374 |
| NCBI Gene ID: | 476 |
| NCBI Accession: | P05023.1 |
| UniProt Secondary Accession: | P05023,Q16689, Q6LDM4, Q9UCN1, Q9UJ20, Q9UJ21, B2RBR6 B7Z2T5, B7Z3U6, F5H3A1, |
| UniProt Related Accession: | P05023 |
| Molecular Weight: | 113kDa |
| NCBI Full Name: | Sodium/potassium-transporting ATPase subunit alpha-1 |
| NCBI Synonym Full Names: | ATPase Na+/K+ transporting subunit alpha 1 |
| NCBI Official Symbol: | ATP1A1 |
| NCBI Protein Information: | sodium/potassium-transporting ATPase subunit alpha-1 |
| UniProt Protein Name: | Sodium/potassium-transporting ATPase subunit alpha-1 |
| UniProt Synonym Protein Names: | Sodium pump subunit alpha-1 |
| Protein Family: | Sodium/potassium-transporting ATPase |
| UniProt Gene Name: | ATP1A1 |
 | Western blot analysis of extracts of various cell lines, using Na+/K+-ATPase Rabbit pAb (CAB7878) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using Na+/K+-ATPase antibody (CAB7878) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using Na+/K+-ATPase antibody (CAB7878) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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