Signal Transduction Antibodies 1
Anti-Na+/K+-ATPase Antibody (CAB12405)
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- SKU:
- CAB12405
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-Na+/K+-ATPase Antibody |
| Antibody SKU: | CAB12405 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 551-850 of human Na+/K+-ATPase (NP_000692.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | THP-1, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 551-850 of human Na+/K+-ATPase (NP_000692.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RVLG FCHL FLPD EQFP EGFQ FDTD DVNF PIDN LCFV GLIS MIDP PRAA VPDA VGKC RSAG IKVI MVTG DHPI TAKA IAKG VGII SEGN ETVE DIAA RLNI PVSQ VNPR DAKA CVVH GSDL KDMT SEQL DDIL KYHT EIVF ARTS PQQK LIIV EGCQ RQGA IVAV TGDG VNDS PALK KADI GVAM GIAG SDVS KQAA DMIL LDDN FASI VTGV EEGR LIFD NLKK SIAY TLTS NIPE ITPF LIFI IANI PLPL GTVT ILCI DLGT DMVP AISL AYEQ AESD IMKR QPRN PKTD KLVN ERLI |
| Gene ID: | 476 |
| Uniprot: | P05023 |
| Cellular Location: | Cell membrane, Melanosome, Multi-pass membrane protein |
| Calculated MW: | 74kDa/109kDa/112kDa/113kDa |
| Observed MW: | 113kDa |
| Synonyms: | ATP1A1, ATPase Na+/K+ transporting subunit alpha 1, ATP1A, CMT2DD, HOMGSMR2 |
| Background: | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ATP1A1: the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients. Two splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:EC 3.6.3.9; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel Chromosomal Location of Human Ortholog: 1p13.1 Cellular Component: endoplasmic reticulum; Golgi apparatus; integral to membrane; membrane; plasma membrane; protein complex; sarcolemma; sodium:potassium-exchanging ATPase complex Molecular Function:ATP binding; chaperone binding; potassium ion binding; protein binding; sodium ion binding; sodium:potassium-exchanging ATPase activity Biological Process: cellular potassium ion homeostasis; cellular sodium ion homeostasis; potassium ion import; regulation of sodium ion transport |
| NCBI Summary: | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| UniProt Code: | P05023 |
| NCBI GenInfo Identifier: | 114374 |
| NCBI Gene ID: | 476 |
| NCBI Accession: | P05023.1 |
| UniProt Secondary Accession: | P05023,Q16689, Q6LDM4, Q9UCN1, Q9UJ20, Q9UJ21, B2RBR6 B7Z2T5, B7Z3U6, F5H3A1, |
| UniProt Related Accession: | P05023 |
| Molecular Weight: | 113kDa |
| NCBI Full Name: | Sodium/potassium-transporting ATPase subunit alpha-1 |
| NCBI Synonym Full Names: | ATPase Na+/K+ transporting subunit alpha 1 |
| NCBI Official Symbol: | ATP1A1 |
| NCBI Protein Information: | sodium/potassium-transporting ATPase subunit alpha-1 |
| UniProt Protein Name: | Sodium/potassium-transporting ATPase subunit alpha-1 |
| UniProt Synonym Protein Names: | Sodium pump subunit alpha-1 |
| Protein Family: | Sodium/potassium-transporting ATPase |
| UniProt Gene Name: | ATP1A1 |
 | Western blot analysis of extracts of various cell lines, using Na+/K+-ATPase1 antibody (CAB12405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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