Antibody Name:	Anti-N6AMT1 Antibody
Antibody SKU:	CAB7201
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human N6AMT1 (NP_877426.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, SKOV3, Mouse heart, Mouse eye, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human N6AMT1 (NP_877426.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGE NFAT PFHG HVGR GAFS DVYE PAED TFLL LNAL EAAA AELA GVEI CLEV GSGS GVVS AFLA SMIG PQAL YMCT DINP EAAA CTLE TARC NKVH IQPV ITDL VGSH GIEA AWAG GRNG REVM DRFF PLVP DLLS PRGL FYLV TIKE NNPE EILK IMKT KGLQ GTTA LSRQ AGQE TLSV LKFT KS
Gene ID:	29104
Uniprot:	Q9Y5N5
Cellular Location:
Calculated MW:	19kDa/22kDa
Observed MW:	23kDa

Synonyms:

N6AMT1, C21orf127, HEMK2, MTQ2, N6AMT, PRED28, m.HsaHemK2P

Background:

This gene encodes an N(6)-adenine-specific DNA methyltransferase. The encoded enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternative splicing pf this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 11.

UniProt Protein Function:	N6AMT1: Heterodimeric methyltransferase that catalyzes N5- methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated. May play a role in the modulation of arsenic-induced toxicity. May be involved in the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid. Belongs to the eukaryotic/archaeal PrmC-related family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.1.1.-; Methyltransferase Chromosomal Location of Human Ortholog: 21q21.3 Cellular Component: protein complex Molecular Function:protein binding; nucleic acid binding; protein methyltransferase activity Biological Process: protein amino acid methylation; positive regulation of cell growth
NCBI Summary:	This gene encodes an N(6)-adenine-specific DNA methyltransferase. The encoded enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternative splicing pf this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Jul 2014]
UniProt Code:	Q9Y5N5
NCBI GenInfo Identifier:	313104228
NCBI Gene ID:	29104
NCBI Accession:	Q9Y5N5.3
UniProt Secondary Accession:	Q9Y5N5,Q96F73,
UniProt Related Accession:	Q9Y5N5
Molecular Weight:	Calculated MW: 19kDa/22kDaObserved MW: 23kDa
NCBI Full Name:	HemK methyltransferase family member 2
NCBI Synonym Full Names:	N-6 adenine-specific DNA methyltransferase 1 (putative)
NCBI Official Symbol:	N6AMT1
NCBI Official Synonym Symbols:	MTQ2; HEMK2; N6AMT; PRED28; C21orf127; m.HsaHemK2P
NCBI Protein Information:	hemK methyltransferase family member 2; N6-DNA-methyltransferase; n(6)-adenine-specific DNA methyltransferase 1
UniProt Protein Name:	HemK methyltransferase family member 2
UniProt Synonym Protein Names:	M.HsaHemK2P; N(6)-adenine-specific DNA methyltransferase 1
Protein Family:	HemK methyltransferase family
UniProt Gene Name:	N6AMT1
UniProt Entry Name:	HEMK2_HUMAN

	Western blot analysis of extracts of various cell lines, using N6AMT1 antibody (CAB7201) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using N6AMT1 Antibody (CAB7201) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney using N6AMT1 Antibody (CAB7201) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using N6AMT1 antibody (CAB7201). Blue: DAPI for nuclear staining.