Cell Biology Antibodies 12
Anti-N4BP1 Antibody (CAB8474)
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- SKU:
- CAB8474
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-N4BP1 Antibody |
| Antibody SKU: | CAB8474 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-590 of mouse N4BP1 (NP_085040.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Jurkat, 293T, Mouse brain, Mouse pancreas |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-590 of mouse N4BP1 (NP_085040.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TQGE ESLF ETDD DVIT VGDV RPPE YTQS AATG PSSA RDEV VVQE DSRN KART PVSE LTKH MDTV FSSS PDVL FVPV NGLS PDED ALSK DRVC HKRR SSDT EERH TKKQ FSLE NVPE GELL PDGK GSAG NEVI DLSD PASN STNL SPDG KDTT EEME YNIL VNFF KTMG YSQE IVEK VIRE YGPS TEPL LLLE EIEK ENKR LQED RDFP PCTV YPDA SQSR NAGV GSTT NELT ADST PKKA QSHT EQSM VERF SQLP FKDS KHCT SNCK VNSF RTVP VGQK QEIW GSKQ NSSC TVDL ETDG HSAS AASA SPKD ISFV SRGA SGHQ QRNP AFPE NGFQ QQTE PLLP NNTK PACE KRSG SCSS PQPK PNYP PLSP PLPL PQLL PSVT EARL GGSS DHID SSVT G |
| Gene ID: | 80750 |
| Uniprot: | O75113 |
| Cellular Location: | Nucleus, PML body, nucleolus |
| Calculated MW: | 99kDa |
| Observed MW: | 120kDa |
| Synonyms: | N4BP1 |
| Background: |
| UniProt Protein Function: | Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. |
| UniProt Code: | O75113 |
| NCBI GenInfo Identifier: | 48928019 |
| NCBI Gene ID: | 9683 |
| NCBI Accession: | NP_694574.3 |
| UniProt Secondary Accession: | O75113,Q2YDX1, A7MD49, |
| UniProt Related Accession: | O75113 |
| Molecular Weight: | 100 KD |
| NCBI Full Name: | NEDD4-binding protein 1 |
| NCBI Synonym Full Names: | NEDD4 binding protein 1 |
| NCBI Official Symbol: | N4BP1 |
| NCBI Protein Information: | NEDD4-binding protein 1 |
| UniProt Protein Name: | NEDD4-binding protein 1 |
| Protein Family: | NEDD4-binding protein |
| UniProt Gene Name: | N4BP1 |
 | Western blot analysis of extracts of various cell lines, using N4BP1 antibody (CAB8474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using N4BP1 antibody (CAB8474) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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