KO Validated Antibodies 2

Anti-N-Cadherin Antibody [KO Validated] (CAB19083)

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SKU:
CAB19083
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-N-Cadherin Antibody [KO Validated]
Antibody SKU:CAB19083
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human N Cadherin
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, NIH/3T3, C6
Immunogen:A synthesized peptide derived from human N Cadherin
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1000
Uniprot:P19022
Cellular Location:Cell membrane, Single-pass type I membrane protein
Calculated MW:140kDa
Observed MW:140kDa
Anti-N-Cadherin Antibody [KO Validated] (CAB19083)Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (CAB19083) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min.
Anti-N-Cadherin Antibody [KO Validated] (CAB19083)Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (CAB19083) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min.
Anti-N-Cadherin Antibody [KO Validated] (CAB19083)Immunohistochemistry of paraffin-embedded mouse heart using N-Cadherin Rabbit mAb (CAB19083) at dilution of 1:100 (40x lens).
Anti-N-Cadherin Antibody [KO Validated] (CAB19083)Immunohistochemistry of paraffin-embedded rat heart using N-Cadherin Rabbit mAb (CAB19083) at dilution of 1:100 (40x lens).
Anti-N-Cadherin Antibody [KO Validated] (CAB19083)Immunofluorescence analysis of mouse heart using N-Cadherin Rabbit mAb (CAB19083) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Synonyms:CD325, CDHN, CDw325, NCAD, N Cadherin, CDH2
Background:This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
UniProt Protein Function:CDH2: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. Interacts with CDCP1. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Interacts with PCDH8; this complex may also include TAOK2. The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: cell-cell adherens junction; focal adhesion; lamellipodium; apical plasma membrane; integral to membrane; plasma membrane; synapse; catenin complex; intercellular junction; fascia adherens

Molecular Function:protein binding; gamma-catenin binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; alpha-catenin binding

Biological Process: heterophilic cell adhesion; intercellular junction assembly and maintenance; muscle cell differentiation; cell migration; glial cell differentiation; positive regulation of MAPKKK cascade; calcium-dependent cell-cell adhesion; blood vessel morphogenesis; positive regulation of muscle cell differentiation; striated muscle cell differentiation; cell adhesion; homophilic cell adhesion

NCBI Summary:This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq, Jul 2008]
UniProt Code:P19022
NCBI GenInfo Identifier:116241277
NCBI Gene ID:1000
NCBI Accession:P19022.4
UniProt Secondary Accession:P19022,Q14923, Q8N173, A8MWK3, B0YIY6,
UniProt Related Accession:P19022
Molecular Weight:97,040 Da
NCBI Full Name:Cadherin-2
NCBI Synonym Full Names:cadherin 2, type 1, N-cadherin (neuronal)
NCBI Official Symbol:CDH2  
NCBI Official Synonym Symbols:CDHN; NCAD; CD325; CDw325  
NCBI Protein Information:cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal
UniProt Protein Name:Cadherin-2
UniProt Synonym Protein Names:CDw325; Neural cadherin; N-cadherin; CD_antigen: CD325
Protein Family:Cadherin
UniProt Gene Name:CDH2  
UniProt Entry Name:CADH2_HUMAN
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