Epigenetics & Nuclear Signaling Antibodies 3

Anti-MYSM1 Antibody (CAB3102)

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SKU:
CAB3102
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-MYSM1 Antibody
Antibody SKU:CAB3102
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human MYSM1 (NP_001078956.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, A-431, HepG2, HeLa, Jurkat, Mouse brain, Rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human MYSM1 (NP_001078956.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PLPY SQIT CLVI SEEI SPDG SYRL PYKF EVQQ MLEE PQWG LVFE KTRW IIEK YRLS HSSV PMDK IFRR DSDL TCLQ KLLE CMRK TLSK VTNC FMAE EFLT EIEN LFLS NYKS NQEN GVTE ENCT KELL M
Gene ID:114803
Uniprot:Q5VVJ2
Cellular Location:Nucleus
Calculated MW:26kDa/66kDa/95kDa
Observed MW:105kDa
Synonyms:MYSM1, 2A-DUB, 2ADUB
Background:
UniProt Protein Function:MYSM1: Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. Belongs to the peptidase M67A family. MYSM1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin-specific protease; EC 3.4.19.-; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p32.1

Cellular Component: nucleoplasm; microtubule cytoskeleton; nucleus

Molecular Function:DNA binding; metallopeptidase activity; histone binding; metal ion binding; ubiquitin-specific protease activity; transcription coactivator activity; protein complex binding; chromatin binding

Biological Process: chromatin remodeling; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; proteolysis

UniProt Code:Q5VVJ2
NCBI GenInfo Identifier:74756898
NCBI Gene ID:114803
NCBI Accession:Q5VVJ2.1
UniProt Secondary Accession:Q5VVJ2,Q68DD3, Q6AI53, Q7Z3G8, Q96PX3, A8KA54, B3KX65
UniProt Related Accession:Q5VVJ2
Molecular Weight:828
NCBI Full Name:Histone H2A deubiquitinase MYSM1
NCBI Synonym Full Names:Myb-like, SWIRM and MPN domains 1
NCBI Official Symbol:MYSM1  
NCBI Official Synonym Symbols:2ADUB; 2A-DUB  
NCBI Protein Information:histone H2A deubiquitinase MYSM1; myb-like, SWIRM and MPN domain-containing protein 1
UniProt Protein Name:Histone H2A deubiquitinase MYSM1
UniProt Synonym Protein Names:Myb-like, SWIRM and MPN domain-containing protein 1
Protein Family:Histone H2A deubiquitinase
UniProt Gene Name:MYSM1  
UniProt Entry Name:MYSM1_HUMAN
Anti-MYSM1 Antibody (CAB3102)Western blot analysis of extracts of various cell lines, using MYSM1 antibody (CAB3102) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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