Name: Anti-MYOG Antibody (CAB17427)
Brand: Cell Cycle Antibodies 1
SKU: CAB17427
Availability: OutOfStock

Antibody Name:	Anti-MYOG Antibody
Antibody SKU:	CAB17427
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-224 of human MYOG (NP_002470.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-224 of human MYOG (NP_002470.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MELY ETSP YFYQ EPRF YDGE NYLP VHLQ GFEP PGYE RTEL TLSP EAPG PLED KGLG TPEH CPGQ CLPW ACKV CKRK SVSV DRRR AATL REKR RLKK VNEA FEAL KRST LLNP NQRL PKVE ILRS AIQY IERL QALL SSLN QEER DLRY RGGG GPQP GVPS ECSS HSAS CSPE WGSA LEFS ANPG DHLL TADP TDAH NLHS LTSI VDSI TVED VSVA FPDE TMPN
Gene ID:	4656
Uniprot:	P15173
Cellular Location:	Nucleus
Calculated MW:	25kDa
Observed MW:	35kDa

Synonyms:	MYOG, MYF4, bHLHc3, myf-4, myogenin, Myogenin
Background:	Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle.

UniProt Protein Function:	myogenin: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 1q32.1 Cellular Component: nucleoplasm; nucleus; transcription factor complex Molecular Function:chromatin DNA binding; protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity Biological Process: mRNA transcription from RNA polymerase II promoter; muscle cell fate commitment; negative regulation of cell proliferation; positive regulation of muscle atrophy; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; regulation of satellite cell proliferation; response to denervation involved in regulation of muscle adaptation; response to electrical stimulus involved in regulation of muscle adaptation; response to muscle activity involved in regulation of muscle adaptation; skeletal muscle development; striated muscle atrophy
NCBI Summary:	Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]
UniProt Code:	P15173
NCBI GenInfo Identifier:	127625
NCBI Gene ID:	4656
NCBI Accession:	P15173.2
UniProt Secondary Accession:	P15173,Q53XW6,
UniProt Related Accession:	P15173
Molecular Weight:	25,037 Da
NCBI Full Name:	Myogenin
NCBI Synonym Full Names:	myogenin
NCBI Official Symbol:	MYOG
NCBI Official Synonym Symbols:	MYF4; myf-4; bHLHc3
NCBI Protein Information:	myogenin
UniProt Protein Name:	Myogenin
UniProt Synonym Protein Names:	Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4
Protein Family:	Myosin
UniProt Gene Name:	MYOG

	Western blot analysis of extracts of Rat skeletal muscle, using MYOG Rabbit pAb (CAB17427) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of NIH/3T3 cells using MYOG antibody (CAB17427) at dilution of 1:100. Blue: DAPI for nuclear staining.