KO Validated Antibodies 2

Anti-MYOF Antibody (CAB19882)[KO Validated]

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SKU:
CAB19882
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MYOF Antibody [KO Validated]
Antibody SKU:CAB19882
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human MYOF.
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa
Immunogen:Recombinant protein of human MYOF.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:26509
Uniprot:Q9NZM1
Cellular Location:Cell membrane, Cytoplasmic vesicle membrane, Nucleus membrane, Single-pass type II membrane protein
Calculated MW:17kDa/46kDa/49kDa/179kDa/229kDa/233kDa/234kDa
Observed MW:260KDa
Anti-MYOF Antibody [KO Validated] (CAB19882)Western blot analysis of extracts from normal (control) and MYOF knockout (KO) HeLa cells, using MYOF antibody (CAB19882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:MYOF, FER1L3, myoferlin
Background:Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined.
UniProt Protein Function:myoferlin: Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR. Belongs to the ferlin family. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nuclear membrane; cytoplasmic vesicle membrane; intracellular membrane-bound organelle; integral to membrane; plasma membrane; nuclear envelope; caveola; cytoplasmic vesicle

Molecular Function:protein binding; phospholipid binding

Biological Process: muscle contraction; plasma membrane repair; blood circulation; regulation of vascular endothelial growth factor receptor signaling pathway; myoblast fusion

NCBI Summary:Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined. [provided by RefSeq, Dec 2008]
UniProt Code:Q9NZM1
NCBI GenInfo Identifier:20139241
NCBI Gene ID:26509
NCBI Accession:Q9NZM1.1
UniProt Related Accession:Q9NZM1
Molecular Weight:Predicted MW: 62.4kDaAccurate MW: 62/44kDa
NCBI Full Name:Myoferlin
NCBI Synonym Full Names:myoferlin
NCBI Official Symbol:MYOF  
NCBI Official Synonym Symbols:FER1L3  
NCBI Protein Information:myoferlin
UniProt Protein Name:Myoferlin
UniProt Synonym Protein Names:Fer-1-like protein 3
Protein Family:Myosin
UniProt Gene Name:MYOF  
UniProt Entry Name:MYOF_HUMAN
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