Developmental Biology
Anti-MYOD1 Antibody (CAB0671)
- SKU:
- CAB0671
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-MYOD1 Antibody |
| Antibody SKU: | CAB0671 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human MYOD1 (NP_002469.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IF 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HT-1080 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human MYOD1 (NP_002469.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LRNA IRYI EGLQ ALLR DQDA APPG AAAA FYAP GPLP PGRG GEHY SGDS DASS PRSN CSDG MMDY SGPP SGAR RRNC YEGA YYNE APSE PRPG KSAA VSSL D |
| Gene ID: | 4654 |
| Uniprot: | P15172 |
| Cellular Location: | Nucleus |
| Calculated MW: | 34kDa |
| Observed MW: | 40KDa |
| Synonyms: | MYOD1, MYF3, MYOD, PUM, bHLHc1 |
| Background: | This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. |
| UniProt Protein Function: | MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor; RNA splicing Chromosomal Location of Human Ortholog: 11p15.4 Cellular Component: nucleoplasm; transcription factor complex; myofibril; cytoplasm; nuclear chromatin; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding Biological Process: transcription from RNA polymerase II promoter; muscle development; skeletal muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; skeletal muscle fiber development; myotube cell development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome |
| NCBI Summary: | This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P15172 |
| NCBI GenInfo Identifier: | 209572729 |
| NCBI Gene ID: | 4654 |
| NCBI Accession: | P15172.3 |
| UniProt Secondary Accession: | P15172,O75321, |
| UniProt Related Accession: | P15172 |
| Molecular Weight: | 320 |
| NCBI Full Name: | Myoblast determination protein 1 |
| NCBI Synonym Full Names: | myogenic differentiation 1 |
| NCBI Official Symbol: | MYOD1 |
| NCBI Official Synonym Symbols: | PUM; MYF3; MYOD; bHLHc1 |
| NCBI Protein Information: | myoblast determination protein 1; myf-3; myogenic factor 3; class C basic helix-loop-helix protein 1 |
| UniProt Protein Name: | Myoblast determination protein 1 |
| UniProt Synonym Protein Names: | Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3 |
| Protein Family: | Myoblast determination protein |
| UniProt Gene Name: | MYOD1 |
| UniProt Entry Name: | MYOD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYOD1 Rabbit pAb (CAB0671) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using MYOD1 antibody (CAB0671) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L-929 cells using MYOD1 antibody (CAB0671) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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